| Genetic Diversity Assessment of Rarely Cultivated Traditional Indica Rice (Oryza sativa L.) Varieties. | |
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MedLine Citation:
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PMID: 21776396 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Random amplified polymorphic DNA fingerprinting was performed to assess the genetic diversity among rarely cultivated traditional indica rice (Oryza sativa L.) varieties collected from a tribal hamlet of Kerala State, India. A total of 664 DNA bands amplified by 15 primers exhibited 72.9% polymorphism (an average of 32.3 polymorphic bands per primer). The varieties Jeerakasala and Kalladiyaran exhibited the highest percent (50.19%) polymorphism, while Thondi and Adukkan showed the lowest (9.85%). Adukkan (78 bands) and Jeerakasala (56 bands) yielded the highest and the lowest number of amplicons, respectively. Unweighted Pair Group Method with Arithmetic mean analysis using the Dice similarity coefficient showed the highest value of similarity coefficient between the varieties Adukkan and Thondi, both shared higher level of similarity (0.81), followed by Kanali and Thondi (0.88). Of the three subclusters, the varieties of Adukkan, Thondi, Kanali, Mannuveliyan, Thonnuranthondi, and Chennellu grouped together with a similarity of 0.77. The second group represented by Navara, Gandhakasala, and Jeerakasala with a similarity coefficient of 0.76 formed a cohesive group. The variety Kalladiyaran formed an isolated position that joined the second cluster. The Principal Coordinate Analysis also showed separation of Kalladiyaran from the other varieties. |
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Authors:
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T Rekha; Kottackal Poulose Martin; V B Sreekumar; Joseph Madassery |
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Publication Detail:
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Type: Journal Article Date: 2011-07-12 |
Journal Detail:
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Title: Biotechnology research international Volume: 2011 ISSN: 2090-3146 ISO Abbreviation: Biotechnol Res Int Publication Date: 2011 |
Date Detail:
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Created Date: 2011-07-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101552363 Medline TA: Biotechnol Res Int Country: England |
Other Details:
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Languages: eng Pagination: 784719 Citation Subset: - |
Affiliation:
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Department of Biotechnology, Calicut University, Kerala 673 635, India. |
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