Document Detail


Genetic Cre-loxP assessment of epicardial cell fate using Wt1-driven Cre alleles.
MedLine Citation:
PMID:  23139287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: Wt1-Cre-based tools are important reagents for studying epicardial cell fate and gene function.
OBJECTIVE: To better describe the properties of Wt1-Cre-based tools to enhance their use in Cre-loxP-based experiments.
METHODS AND RESULTS: In contrast to recently reported results, we show that constitutive Wt1(GFPCre) in combination with certain Cre-activated reporters can be used to trace (pro) epicardial cell fate. Wt1(CreERT2) can be efficiently induced by tamoxifen administration. We show substantial labeling of coronary endothelial cells when induction is performed at late but not early stages of heart development.
CONCLUSIONS: Wt1-based Cre alleles are useful tools for genetic lineage tracing of epicardial cells and mesothelium of other organs. Using these tools with proper understanding of their properties and limitations enables genetic labeling of epicardial cells and their derivatives.
Authors:
Bin Zhou; William T Pu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation research     Volume:  111     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-02-05     Revised Date:  2013-11-14    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e276-80     Citation Subset:  IM    
Affiliation:
Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. zhoubin@sibs.ac.cn
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Cell Lineage*
Embryo, Mammalian / cytology*,  drug effects,  metabolism
Endothelial Cells / metabolism
Estrogen Antagonists / pharmacology
Green Fluorescent Proteins / genetics,  metabolism
Immunohistochemistry
Integrases / genetics
Mice
Mice, Transgenic
Pericardium / cytology*,  embryology,  metabolism
Tamoxifen / pharmacology
Time Factors
WT1 Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 HL094683/HL/NHLBI NIH HHS; R01 HL094683/HL/NHLBI NIH HHS; U01 HL100401/HL/NHLBI NIH HHS; U01HL100401/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Estrogen Antagonists; 0/WT1 Proteins; 10540-29-1/Tamoxifen; 147336-22-9/Green Fluorescent Proteins; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases
Comments/Corrections

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