Document Detail


Genetic architecture of ambulatory blood pressure in the general population: insights from cardiovascular gene-centric array.
MedLine Citation:
PMID:  21060006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetic determinants of blood pressure are poorly defined. We undertook a large-scale, gene-centric analysis to identify loci and pathways associated with ambulatory systolic and diastolic blood pressure. We measured 24-hour ambulatory blood pressure in 2020 individuals from 520 white European nuclear families (the Genetic Regulation of Arterial Pressure of Humans in the Community Study) and genotyped their DNA using the Illumina HumanCVD BeadChip array, which contains ≈ 50 000 single nucleotide polymorphisms in >2000 cardiovascular candidate loci. We found a strong association between rs13306560 polymorphism in the promoter region of MTHFR and CLCN6 and mean 24-hour diastolic blood pressure; each minor allele copy of rs13306560 was associated with 2.6 mm Hg lower mean 24-hour diastolic blood pressure (P = 1.2 × 10⁻⁸). rs13306560 was also associated with clinic diastolic blood pressure in a combined analysis of 8129 subjects from the Genetic Regulation of Arterial Pressure of Humans in the Community Study, the CoLaus Study, and the Silesian Cardiovascular Study (P=5.4 × 10⁻⁶). Additional analysis of associations between variants in gene ontology-defined pathways and mean 24-hour blood pressure in the Genetic Regulation of Arterial Pressure of Humans in the Community Study showed that cell survival control signaling cascades could play a role in blood pressure regulation. There was also a significant overrepresentation of rare variants (minor allele frequency: < 0.05) among polymorphisms showing at least nominal association with mean 24-hour blood pressure indicating that a considerable proportion of its heritability may be explained by uncommon alleles. Through a large-scale gene-centric analysis of ambulatory blood pressure, we identified an association of a novel variant at the MTHFR/CLNC6 locus with diastolic blood pressure and provided new insights into the genetic architecture of blood pressure.
Authors:
Maciej Tomaszewski; Radoslaw Debiec; Peter S Braund; Christopher P Nelson; Robert Hardwick; Paraskevi Christofidou; Matthew Denniff; Veryan Codd; Suzanne Rafelt; Pim van der Harst; Dawn Waterworth; Kijoung Song; Peter Vollenweider; Gerard Waeber; Ewa Zukowska-Szczechowska; Paul R Burton; Vincent Mooser; Fadi J Charchar; John R Thompson; Martin D Tobin; Nilesh J Samani
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-11-08
Journal Detail:
Title:  Hypertension     Volume:  56     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-18     Completed Date:  2010-12-17     Revised Date:  2011-12-21    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1069-76     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Sciences, University of Leicester, Clinical Sciences Wing, Glenfield Hospital, Leicester, UK. njs@le.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Blood Pressure / genetics*
Blood Pressure Monitoring, Ambulatory*
Chloride Channels / genetics*
Cohort Studies
European Continental Ancestry Group / genetics
Female
Gene Frequency
Genetic Association Studies
Genetic Loci
Genotype
Great Britain / epidemiology
Humans
Hypertension / epidemiology*,  genetics*
Male
Methylenetetrahydrofolate Reductase (NADPH2) / genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis
Polymorphism, Single Nucleotide
Prevalence
Promoter Regions, Genetic
Young Adult
Grant Support
ID/Acronym/Agency:
R03 TW007165/TW/FIC NIH HHS; R03 TW007165-03/TW/FIC NIH HHS; //British Heart Foundation
Chemical
Reg. No./Substance:
0/CLCN6 protein, human; 0/Chloride Channels; EC 1.5.1.20/Methylenetetrahydrofolate Reductase (NADPH2)
Comments/Corrections
Comment In:
Hypertension. 2010 Dec;56(6):1035-7   [PMID:  21060002 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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