Document Detail

Genetic ablation of CD36 does not alter mouse brain polyunsaturated fatty acid concentrations.
MedLine Citation:
PMID:  20306148     Owner:  NLM     Status:  MEDLINE    
In the brain, polyunsaturated fatty acids (PUFA), especially arachidonic acid and docosahexaenoic acid (DHA), are required for regulating membrane fluidity, neuronal survival and signal transduction. Since the brain cannot synthesize n-6 and n-3 PUFA de novo, they must be supplied from the blood. However, the methods of PUFA entry into the brain are not agreed upon. This study tested the necessity of CD36, a candidate transporter of unesterified fatty acids, for maintaining brain PUFA concentrations by comparing brain PUFA concentrations in CD36(-/-) mice to their wild-type littermates. Because CD36(-/-) mice have been reported to have impaired learning ability, the PUFA concentrations in different brain regions (cortex, hippocampus, cerebellum and the remainder of brain) were investigated. At 9 weeks of age, the brain was separated into the four regions and fatty acid concentrations in total and phospholipid classes of these brain regions were analyzed using thin layer and gas chromatography. There were no statistical differences in arachidonic acid or DHA concentrations in the different brain regions between wild-type and CD36(-/-) mice, in total or phospholipid fractions. Concentrations of monounsaturated fatty acids were decreased in several phospholipid fractions in CD36(-/-) mice. These findings suggest that CD36 is not necessary for maintaining brain PUFA concentrations and that other mechanisms must exist.
Byung Jun Song; Adrienne Elbert; Tupur Rahman; Sarah K Orr; Chuck T Chen; Maria Febbraio; Richard P Bazinet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-20
Journal Detail:
Title:  Lipids     Volume:  45     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  291-9     Citation Subset:  IM    
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, FitzGerald Building, 150 College St., Room 306, Toronto, ON M5S3E2, Canada.
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MeSH Terms
Antigens, CD36 / genetics*
Brain / metabolism*
Brain Chemistry / genetics
Cholesterol / analysis,  metabolism
Fatty Acids, Unsaturated / analysis,  metabolism*
Gene Deletion*
Lipid Metabolism / genetics
Mice, Inbred C57BL
Mice, Knockout
Osmolar Concentration
Reg. No./Substance:
0/Antigens, CD36; 0/Fatty Acids, Unsaturated; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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