| Genetic ablation of CD36 does not alter mouse brain polyunsaturated fatty acid concentrations. | |
| | |
MedLine Citation:
|
PMID: 20306148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the brain, polyunsaturated fatty acids (PUFA), especially arachidonic acid and docosahexaenoic acid (DHA), are required for regulating membrane fluidity, neuronal survival and signal transduction. Since the brain cannot synthesize n-6 and n-3 PUFA de novo, they must be supplied from the blood. However, the methods of PUFA entry into the brain are not agreed upon. This study tested the necessity of CD36, a candidate transporter of unesterified fatty acids, for maintaining brain PUFA concentrations by comparing brain PUFA concentrations in CD36(-/-) mice to their wild-type littermates. Because CD36(-/-) mice have been reported to have impaired learning ability, the PUFA concentrations in different brain regions (cortex, hippocampus, cerebellum and the remainder of brain) were investigated. At 9 weeks of age, the brain was separated into the four regions and fatty acid concentrations in total and phospholipid classes of these brain regions were analyzed using thin layer and gas chromatography. There were no statistical differences in arachidonic acid or DHA concentrations in the different brain regions between wild-type and CD36(-/-) mice, in total or phospholipid fractions. Concentrations of monounsaturated fatty acids were decreased in several phospholipid fractions in CD36(-/-) mice. These findings suggest that CD36 is not necessary for maintaining brain PUFA concentrations and that other mechanisms must exist. |
| | |
Authors:
|
Byung Jun Song; Adrienne Elbert; Tupur Rahman; Sarah K Orr; Chuck T Chen; Maria Febbraio; Richard P Bazinet |
Related Documents
:
|
6183268 - Bovine fibroblast growth factor: comparison of brain and pituitary preparations. 7457718 - Blood-brain barrier derangement in sepsis: cause of septic encephalopathy? 530468 - In vivo incorporation of exogenous [1-14c]stearic acid into neurons and astrocytes. 17107928 - Targeting the cerebrovascular large neutral amino acid transporter (lat1) isoform using... 15581858 - The effect of merulinic acid on biomembranes. 14488628 - Utilization of sulfur compounds by streptococcus bovis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-20 |
Journal Detail:
|
Title: Lipids Volume: 45 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-03-25 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
|
Languages: eng Pagination: 291-9 Citation Subset: IM |
Affiliation:
|
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, FitzGerald Building, 150 College St., Room 306, Toronto, ON M5S3E2, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD36 / genetics* Brain / metabolism* Brain Chemistry / genetics Cholesterol / analysis, metabolism Fatty Acids, Unsaturated / analysis, metabolism* Gene Deletion* Lipid Metabolism / genetics Mice Mice, Inbred C57BL Mice, Knockout Osmolar Concentration |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD36; 0/Fatty Acids, Unsaturated; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Product-form stationary distributions for deficiency zero chemical reaction networks.
Next Document: Dependence on emergency care among young adults in the United States.