Document Detail


Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  11606525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accurate chromosome segregation requires the precise coordination of events during the cell cycle. Replicated sister chromatids are held together while they are properly attached to and aligned by the mitotic spindle at metaphase. At anaphase, the links between sisters must be promptly dissolved to allow the mitotic spindle to rapidly separate them to opposite poles. To isolate genes involved in chromosome behavior during mitosis, we microscopically screened a temperature-sensitive collection of budding yeast mutants that contain a GFP-marked chromosome. Nine LOC (loss of cohesion) complementation groups that do not segregate sister chromatids at anaphase were identified. We cloned the corresponding genes and performed secondary tests to determine their function in chromosome behavior. We determined that three LOC genes, PDS1, ESP1, and YCS4, are required for sister chromatid separation and three other LOC genes, CSE4, IPL1, and SMT3, are required for chromosome segregation. We isolated alleles of two genes involved in splicing, PRP16 and PRP19, which impair alpha-tubulin synthesis thus preventing spindle assembly, as well as an allele of CDC7 that is defective in DNA replication. We also report an initial characterization of phenotypes associated with the SMT3/SUMO gene and the isolation of WSS1, a high-copy smt3 suppressor.
Authors:
S Biggins; N Bhalla; A Chang; D L Smith; A W Murray
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  159     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-18     Completed Date:  2002-01-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  453-70     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of California, San Francisco, 94143, USA. sbiggins@fhcrc.org
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MeSH Terms
Descriptor/Qualifier:
Alleles
Base Sequence
Chromatids*
Chromosomes, Fungal
Cloning, Molecular
DNA Primers
Genes, Fungal*
Genes, Suppressor
Genetic Complementation Test
Mutation
Saccharomyces cerevisiae / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers
Comments/Corrections

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