Document Detail


Genes affecting the activity of nicotinic receptors involved in Caenorhabditis elegans egg-laying behavior.
MedLine Citation:
PMID:  11290716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Egg-laying behavior in Caenorhabditis elegans is regulated by multiple neurotransmitters, including acetylcholine and serotonin. Agonists of nicotinic acetylcholine receptors such as nicotine and levamisole stimulate egg laying; however, the genetic and molecular basis for cholinergic neurotransmission in the egg-laying circuitry is not well understood. Here we describe the egg-laying phenotypes of eight levamisole resistance genes, which affect the activity of levamisole-sensitive nicotinic receptors in nematodes. Seven of these genes, including the nicotinic receptor subunit genes unc-29, unc-38, and lev-1, were essential for the stimulation of egg laying by levamisole, though they had only subtle effects on egg-laying behavior in the absence of drug. Thus, these genes appear to encode components of a nicotinic receptor that can promote egg laying but is not necessary for egg-laying muscle contraction. Since the levamisole-receptor mutants responded to other cholinergic drugs, other acetylcholine receptors are likely to function in parallel with the levamisole-sensitive receptors to mediate cholinergic neurotransmission in the egg-laying circuitry. In addition, since expression of functional unc-29 in muscle cells restored levamisole sensitivity under some but not all conditions, both neuronal and muscle cell UNC-29 receptors are likely to contribute to the regulation of egg-laying behavior. Mutations in one levamisole receptor gene, unc-38, also conferred both hypersensitivity and reduced peak response to serotonin; thus nicotinic receptors may play a role in regulating serotonin response pathways in the egg-laying neuromusculature.
Authors:
J Kim; D S Poole; L E Waggoner; A Kempf; D S Ramirez; P A Treschow; W R Schafer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  157     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-06     Completed Date:  2001-07-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1599-610     Citation Subset:  IM    
Affiliation:
Division of Biology, University of California, San Diego, California 92093-0349, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / genetics,  physiology*
Genes, Helminth / physiology*
Helminth Proteins / genetics,  metabolism
Levamisole / metabolism*
Oviposition / physiology*
Phenotype
Receptors, Nicotinic / genetics*,  metabolism
Serotonin / metabolism
Grant Support
ID/Acronym/Agency:
DA11556/DA/NIDA NIH HHS; DA12891/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Helminth Proteins; 0/Receptors, Nicotinic; 14769-73-4/Levamisole; 50-67-9/Serotonin
Comments/Corrections

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