Document Detail


Genes, behavior and next-generation RNA sequencing.
MedLine Citation:
PMID:  23194347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Advances in next-generation sequencing suggest that RNA-Seq is poised to supplant microarray-based approaches for transcriptome analysis. This article briefly reviews the use of microarrays in the brain-behavior context and then illustrates why RNA-Seq is a superior strategy. Compared with microarrays, RNA-Seq has a greater dynamic range, detects both coding and noncoding RNAs, is superior for gene network construction, detects alternative spliced transcripts, detects allele specific expression and can be used to extract genotype information, e.g. nonsynonymous coding single nucleotide polymorphisms. Examples of where RNA-Seq has been used to assess brain gene expression are provided. Despite the advantages of RNA-Seq, some disadvantages remain. These include the high cost of RNA-Seq and the computational complexities associated with data analysis. RNA-Seq embraces the complexity of the transcriptome and provides a mechanism to understand the underlying regulatory code; the potential to inform the brain-behavior relationship is substantial.
Authors:
R Hitzemann; D Bottomly; P Darakjian; N Walter; O Iancu; R Searles; B Wilmot; S McWeeney
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2012-12-28
Journal Detail:
Title:  Genes, brain, and behavior     Volume:  12     ISSN:  1601-183X     ISO Abbreviation:  Genes Brain Behav.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-23     Completed Date:  2013-07-03     Revised Date:  2013-10-24    
Medline Journal Info:
Nlm Unique ID:  101129617     Medline TA:  Genes Brain Behav     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-12     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Genes, Brain and Behavior © 2012 Blackwell Publishing Ltd and International Behavioural and Neural Genetics Society.
Affiliation:
Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR 97239-3098, USA. hitzeman@ohsu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism*
Gene Expression Profiling*
Genes
High-Throughput Nucleotide Sequencing / methods*
Humans
Oligonucleotide Array Sequence Analysis
Sequence Analysis, RNA / methods*
Grant Support
ID/Acronym/Agency:
AA 10760/AA/NIAAA NIH HHS; AA 11034/AA/NIAAA NIH HHS; AA 13484/AA/NIAAA NIH HHS; MH 51372/MH/NIMH NIH HHS; R01 MH051372/MH/NIMH NIH HHS; U01 AA013484/AA/NIAAA NIH HHS; UL1 TR000128/TR/NCATS NIH HHS

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