Document Detail

Generation of transgenic dairy cattle using 'in vitro' embryo production.
MedLine Citation:
PMID:  1367358     Owner:  NLM     Status:  MEDLINE    
We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.
P Krimpenfort; A Rademakers; W Eyestone; A van der Schans; S van den Broek; P Kooiman; E Kootwijk; G Platenburg; F Pieper; R Strijker
Related Documents :
7710298 - Effect of anesthesia on sperm motility in gamete intrafallopian transfer.
9013308 - The role of uterine straightening by passive bladder distension before embryo transfer ...
1833418 - Delayed embryo transfer in an in-vitro fertilization programme: how to avoid working on...
8671508 - In-vitro fertilization treatment for unexplained recurrent abortion: a pilot study.
16167668 - Low birth weight in south asian babies in britain: time to reduce the inequalities.
8531098 - L(+)- and d(-)-lactate modulate rat renal tubular accumulation of amantadine in the pre...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  9     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  844-7     Citation Subset:  B    
Department of Embryology, Gene Pharming Europe B.V., Leiden, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Genetically Modified*
Blotting, Southern
Caseins / genetics*
Cattle / genetics*
Cells, Cultured
DNA / genetics,  isolation & purification
Embryo Transfer
Embryonic and Fetal Development
Lactoferrin / genetics*
Oocytes / physiology
Restriction Mapping
Reg. No./Substance:
0/Caseins; 0/Lactoferrin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  High level expression of active human alpha-1-antitrypsin in the milk of transgenic sheep.
Next Document:  Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pe...