Document Detail


Generation of transgenic dairy cattle using 'in vitro' embryo production.
MedLine Citation:
PMID:  1367358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.
Authors:
P Krimpenfort; A Rademakers; W Eyestone; A van der Schans; S van den Broek; P Kooiman; E Kootwijk; G Platenburg; F Pieper; R Strijker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  9     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  844-7     Citation Subset:  B    
Affiliation:
Department of Embryology, Gene Pharming Europe B.V., Leiden, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified*
Blotting, Southern
Caseins / genetics*
Cattle / genetics*
Cells, Cultured
DNA / genetics,  isolation & purification
Embryo Transfer
Embryonic and Fetal Development
Female
Humans
Lactoferrin / genetics*
Oocytes / physiology
Pregnancy
Restriction Mapping
Transfection*
Chemical
Reg. No./Substance:
0/Caseins; 0/Lactoferrin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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