| Generation of transgenic Xenopus using restriction enzyme-mediated integration. | |
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MedLine Citation:
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PMID: 22688696 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Transgenesis, the process of incorporating an exogenous gene (transgene) into an organism's genome, is a widely used tool to develop models of human diseases and to study the function and/or regulation of genes. Generating transgenic Xenopus is rapid and involves simple in vitro manipulations, taking advantage of the large size of the amphibian egg and external embryonic development. Restriction enzyme-mediated integration (REMI) has a number of advantages for transgenesis compared to other methods used to produce transgenic Xenopus, including relative efficiency, higher transgene expression levels, fewer genetic chimera in founder transgenic animals, and near-complete germ-line transgene transmission. This chapter explains the REMI method for generating transgenic Xenopus laevis tadpoles, including improvements developed to enable studies in the mature retina. |
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Authors:
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Mohammad Haeri; Barry E Knox |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 884 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-06-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 17-39 Citation Subset: IM |
Affiliation:
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Department of Neuroscience & Physiology, SUNY Upstate Medical University, Syracuse, NY, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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