Document Detail

Generation of rubella virus-neutralising antibodies by vaccination with synthetic peptides.
MedLine Citation:
PMID:  7539669     Owner:  NLM     Status:  MEDLINE    
Four short peptides from rubella virus proteins E1 and E2, predicted to contain B cell epitopes, were used to vaccinate BALB/c mice. Sera from peptide-vaccinated animals reacted with viral antigens in ELISA and three of the four induced virus-neutralising antibody (nAb) responses. Peptide PY4, in contrast to the others, induced IgG2a responses upon vaccination and stimulated spleen cells in vitro produced IFN gamma in the absence of IL-5. It was reasoned that vaccination with PY4 caused Th1 subset activation, the appropriate type of response for anti-viral immunity and hence the efficient neutralising antibody response. Presentation of peptide for vaccination proved to be as important as the sequence. Similar profiles of IgG1 and IgG2a were detected in the sera of mice vaccinated with PY4 in Freund's complete adjuvant or alum; however nAb responses were not found when alum was used.
K Robinson; A Mostratos; R K Grencis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  10     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-07-13     Completed Date:  1995-07-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
School of Biological Sciences, University of Manchester, UK.
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MeSH Terms
Alum Compounds / pharmacology
Amino Acid Sequence
Antibodies, Viral / biosynthesis*
Enzyme-Linked Immunosorbent Assay
Epitopes / chemistry
Freund's Adjuvant / pharmacology
Immunoglobulin Isotypes / biosynthesis
Interferon-gamma / biosynthesis
Interleukin-5 / biosynthesis
Mice, Inbred BALB C
Molecular Sequence Data
Neutralization Tests
Oligopeptides / immunology*
Rubella Vaccine / immunology*
Rubella virus / immunology*
Vaccines, Synthetic / immunology*
Viral Envelope Proteins / chemistry
Reg. No./Substance:
0/Alum Compounds; 0/Antibodies, Viral; 0/Epitopes; 0/Immunoglobulin Isotypes; 0/Interleukin-5; 0/Oligopeptides; 0/Rubella Vaccine; 0/Vaccines, Synthetic; 0/Viral Envelope Proteins; 10043-01-3/aluminum sulfate; 131016-01-8/E1 envelope protein, Rubella virus; 131016-03-0/E2 envelope protein, Rubella virus; 82115-62-6/Interferon-gamma; 9007-81-2/Freund's Adjuvant

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