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Generation of rice mutants by chemical mutagenesis.
MedLine Citation:
PMID:  23135842     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Chemical mutagenesis of rice has been used extensively to generate useful genetic variation for the purpose of breeding improved varieties. More recently, advances in high-throughput genotyping platforms have enabled the efficient detection of point mutations generated by chemical agents. This in turn has renewed interest in using traditional chemical mutagenesis to generate mutant populations for gene discovery and functional characterization. Targeting of Induced Local Lesions in Genomes (TILLING) is a powerful reverse genetics method which combines chemical mutagenesis with the high-throughput discovery of point mutations. Numerous chemical mutagens have been shown to be effective in generating point mutations and small deletions in rice. This chapter describes the use of a combination of sodium azide (NaN(3)) and N-nitroso-N-methylurea to generate populations that are suitable for TILLING as well as forward genetics and mutation breeding.
Authors:
Thomas H Tai
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  29-37     Citation Subset:  IM    
Affiliation:
USDA-ARS Crops Pathology and Genetics Research Unit and Department of Plant Sciences, University of California, Davis, CA, USA, Thomas.Tai@ars.usda.gov.
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