| Generation of polyclonal antibodies against recombinant human activin A. | |
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MedLine Citation:
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PMID: 1806589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A goat antiserum to purified recombinant human activin A (rhAct-A), a dimer formed by two beta A-subunits of inhibin, has been produced. The immunoreactivity of the antiserum has been evaluated in an antigen coated enzyme-linked immunosorbent assay, in a radioimmunoassay using iodinated rhAct-A, and by Western blot analysis. The antiserum demonstrated some cross reactivity to inhibin A, a structurally related heterodimer which contains an identical beta A-subunit coupled to a distinct, though similar, alpha subunit. A simple radioimmunoassay for rhAct-A in tissue culture supernatant has been developed with rhAct-A affinity column purified polyclonal antiserum. The assay is precise and sensitive with a range of 0.31-40 ng/ml. The cross reactivity of inhibin A in the RIA is about 4.3%. Despite its cross-reactivity this antiserum will facilitate studies of the physiology of activin A and inhibin A which includes a Western blot analysis where a molecular size distinction is accomplished. |
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Authors:
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J A Lofgren; R Schwall; C Schmelzer; W L Wong |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunoassay Volume: 12 ISSN: 0197-1522 ISO Abbreviation: J Immunoassay Publication Date: 1991 |
Date Detail:
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Created Date: 1992-05-08 Completed Date: 1992-05-08 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8007167 Medline TA: J Immunoassay Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 565-78 Citation Subset: IM |
Affiliation:
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Department of Immunology Research & Assay Technology, Genentech Inc., South San Francisco, California 94080. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Activins Animals Antibodies / immunology* Antibody Formation Antibody Specificity Blotting, Western Female Goats / immunology Humans Immunization Inhibins / immunology* Male Rabbits / immunology Radioimmunoassay Recombinant Proteins / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Recombinant Proteins; 104625-48-1/Activins; 57285-09-3/Inhibins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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