Document Detail


Generation of pluripotent stem cells from patients with type 1 diabetes.
MedLine Citation:
PMID:  19720998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Type 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic beta cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallmarks of pluripotency and can be differentiated into insulin-producing cells. These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy.
Authors:
René Maehr; Shuibing Chen; Melinda Snitow; Thomas Ludwig; Lisa Yagasaki; Robin Goland; Rudolph L Leibel; Douglas A Melton
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-31
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  106     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-10-06     Completed Date:  2009-11-13     Revised Date:  2011-09-02    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15768-73     Citation Subset:  IM    
Affiliation:
Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Adult Stem Cells / metabolism,  pathology*,  transplantation
Cell Dedifferentiation
Cell Differentiation
Cells, Cultured
Diabetes Mellitus, Type 1 / metabolism,  pathology*,  therapy
Endoderm / cytology,  metabolism
Germ Layers / cytology,  metabolism
Humans
Male
Models, Biological
Pancreas / cytology
Pluripotent Stem Cells / metabolism,  pathology*,  transplantation
Transcription Factors / metabolism
Grant Support
ID/Acronym/Agency:
R01 DK052431-17/DK/NIDDK NIH HHS; R01 DK052431-18/DK/NIDDK NIH HHS; R01 DK052431-19/DK/NIDDK NIH HHS; R01 DK066518-08/DK/NIDDK NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Transcription Factors
Comments/Corrections
Comment In:
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15523-4   [PMID:  19805208 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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