| Generation of a phenotypic array of hypothalamic neuronal cell models to study complex neuroendocrine disorders. | |
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MedLine Citation:
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PMID: 14551229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Knowledge of how the brain achieves its diverse central control of basic physiology is severely limited by the virtual absence of appropriate cell models. Isolation of clonal populations of unique peptidergic neurons from the hypothalamus will facilitate these studies. Herein we describe the mass immortalization of mouse primary hypothalamic cells in monolayer culture, resulting in the generation of a vast representation of hypothalamic cell types. Subcloning of the heterogeneous cell populations resulted in the establishment of 38 representative clonal neuronal cell lines, of which 16 have been further characterized by analysis of 28 neuroendocrine markers. These cell lines represent the first available models to study the regulation of neuropeptides associated with the control of feeding behavior, including neuropeptide Y, ghrelin, urocortin, proopiomelanocortin, melanin-concentrating hormone, neurotensin, proglucagon, and GHRH. Importantly, a representative cell line responds appropriately to leptin stimulation and results in the repression of neuropeptide Y gene expression. These cell models can be used for detailed molecular analysis of neuropeptide gene regulation and signal transduction events involved in the direct hormonal control of unique hypothalamic neurons, not yet possible in the whole brain. Such studies may contribute information necessary for the strategic design of therapeutic interventions for complex neuroendocrine disorders, such as obesity. |
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Authors:
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Denise D Belsham; Fang Cai; Hong Cui; Simon R Smukler; Anne Marie F Salapatek; Lulzim Shkreta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-10-09 |
Journal Detail:
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Title: Endocrinology Volume: 145 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2003-12-18 Completed Date: 2004-01-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 393-400 Citation Subset: AIM; IM |
Affiliation:
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Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8. d.belsham@utoronto.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Transformed Clone Cells Female Hypothalamic Diseases / genetics*, physiopathology* Hypothalamus / cytology, physiology* Male Mice Mice, Inbred BALB C Neurons / physiology* Oligonucleotide Array Sequence Analysis* Phenotype |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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