| Generation of p53-Deficient Induced Pluripotent Stem Cells from Mouse Embryo Fibroblasts. | |
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MedLine Citation:
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PMID: 23150445 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Here we describe a method for generating induced pluripotent stem (iPS) cells from mouse embryonic fibroblasts (MEFs). Recombinant retroviruses carrying human transcription factors for Klf4, Oct3/4, Sox2, with or without c-Myc, are used to transduce early passage MEFs several times. Based on morphologic criteria, the resulting iPS colonies are picked manually at first, and then propagated and expanded by standard methods. iPS cells can then be differentiated into virtually any cell type or lineage, thus allowing for discoveries of new functions of p53 and mutant p53. |
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Authors:
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Evguenia M Alexandrova; Ute M Moll |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 962 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 157-164 Citation Subset: - |
Affiliation:
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Department of Pathology, Stony Brook University, Stony Brook, NY, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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