| Generation of nitric oxide from S-nitrosothiols using protein-bound Cu2+ sources. | |
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MedLine Citation:
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PMID: 8807899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We have recently shown that S-nitrosothiols (RSNOs) decompose in aqueous buffer to give nitric oxide, an important signalling molecule, and the corresponding disulphides. This occurs by reaction with Cu+ generated from Cu2+ (supplied as hydrated Cu2+) by thiolate reduction. To establish whether these reactions are feasible in vivo, we set out to determine whether Cu2+ bound to an amino acid, a tripeptide or to human serum albumin (HSA) could serve as a Cu+ source for generation of NO from S-nitrosothiols. RESULTS: Experiments with Cu2+ bound to the tripeptide Gly-Gly-His or to two histidine molecules or to HSA showed that Cu+ was released (and trapped with neocuproine) when the copper source was treated with a thiol at pH 7.4. RSNO decomposition was achieved with all three copper sources, although not as rapidly as with added hydrated Cu2+. Decomposition was also catalyzed by ceruloplasmin. CONCLUSIONS: These results show clearly that amino-acid- and protein-bound Cu2+ can be reduced by thiolate ion to Cu+, which will generate NO from RSNO species, thus providing a realistic model for these reactions in vivo. |
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Authors:
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A P Dicks; D L Williams |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Chemistry & biology Volume: 3 ISSN: 1074-5521 ISO Abbreviation: Chem. Biol. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1997-02-19 Completed Date: 1997-02-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9500160 Medline TA: Chem Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 655-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Durham University, Durham DH1 3LE, UK. D.L.H.Williams@durham.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Copper
/
chemistry,
metabolism* Humans Kinetics Nitric Oxide / chemistry, metabolism* Nitroso Compounds / chemistry, metabolism* Oligopeptides / chemistry, metabolism Oxidation-Reduction Protein Binding Serum Albumin / chemistry, metabolism Sulfhydryl Compounds / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Nitroso Compounds; 0/Oligopeptides; 0/Serum Albumin; 0/Sulfhydryl Compounds; 10102-43-9/Nitric Oxide; 7440-50-8/Copper; 7451-76-5/diglycyl-histidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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