Document Detail

Generation of nitric oxide from S-nitrosothiols using protein-bound Cu2+ sources.
MedLine Citation:
PMID:  8807899     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: We have recently shown that S-nitrosothiols (RSNOs) decompose in aqueous buffer to give nitric oxide, an important signalling molecule, and the corresponding disulphides. This occurs by reaction with Cu+ generated from Cu2+ (supplied as hydrated Cu2+) by thiolate reduction. To establish whether these reactions are feasible in vivo, we set out to determine whether Cu2+ bound to an amino acid, a tripeptide or to human serum albumin (HSA) could serve as a Cu+ source for generation of NO from S-nitrosothiols. RESULTS: Experiments with Cu2+ bound to the tripeptide Gly-Gly-His or to two histidine molecules or to HSA showed that Cu+ was released (and trapped with neocuproine) when the copper source was treated with a thiol at pH 7.4. RSNO decomposition was achieved with all three copper sources, although not as rapidly as with added hydrated Cu2+. Decomposition was also catalyzed by ceruloplasmin. CONCLUSIONS: These results show clearly that amino-acid- and protein-bound Cu2+ can be reduced by thiolate ion to Cu+, which will generate NO from RSNO species, thus providing a realistic model for these reactions in vivo.
A P Dicks; D L Williams
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Chemistry & biology     Volume:  3     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-02-19     Completed Date:  1997-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  655-9     Citation Subset:  IM    
Department of Chemistry, Durham University, Durham DH1 3LE, UK.
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MeSH Terms
Copper / chemistry,  metabolism*
Nitric Oxide / chemistry,  metabolism*
Nitroso Compounds / chemistry,  metabolism*
Oligopeptides / chemistry,  metabolism
Protein Binding
Serum Albumin / chemistry,  metabolism
Sulfhydryl Compounds / chemistry,  metabolism*
Reg. No./Substance:
0/Nitroso Compounds; 0/Oligopeptides; 0/Serum Albumin; 0/Sulfhydryl Compounds; 10102-43-9/Nitric Oxide; 7440-50-8/Copper; 7451-76-5/diglycyl-histidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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