Document Detail

Generation of multinucleated giant cells in vitro from bovine monocytes and macrophages.
MedLine Citation:
PMID:  15472469     Owner:  NLM     Status:  MEDLINE    
The generation of multinucleated giant cells (MGC) from cells of the bovine monocyte-macrophage lineage was investigated. Freshly isolated monocytes were incubated with the conditioned medium (CM) of peripheral blood mononuclear cell cultures treated with Concanavalin A for 1-4 days (CM1 to CM4). Only CM1 generated MGC despite similar concentrations of IFNgamma in all CMs. Nevertheless, MGC formation from monocytes was enhanced by adding either macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), MGC formations from macrophages were observed only when macrophages were cultured with GM-CSF plus CM. These results indicate that several mechanisms to generate MGC from bovine monocytes-macrophage lineage cells exist, and that GM-CSF is a major mediator of MGC formation in cattle.
Kazuhiro Yoshihara; Reiko Nagata; Yoshihiro Muneta; Shigeki Inumaru; Yuichi Yokomizo; Yasuyuki Mori
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  66     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-10-08     Completed Date:  2005-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1065-9     Citation Subset:  IM    
National Institute of Animal Health, Tsukuba, Japan.
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MeSH Terms
Cattle / immunology*
Concanavalin A
Giant Cells / cytology,  drug effects*
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
Macrophages / physiology*
Monocytes / physiology*
Phagocytosis / physiology
Reg. No./Substance:
11028-71-0/Concanavalin A; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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