| Generation of a mouse line expressing Cre recombinase in glycinergic interneurons. | |
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MedLine Citation:
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PMID: 20506101 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In caudal regions of the CNS, glycine constitutes the major inhibitory neurotransmitter. Here, we describe a mouse line that expresses Cre recombinase under the control of a BAC transgenic glycine transporter 2 (GlyT2) promoter fragment. Mating of GlyT2-Cre mice with the Cre reporter mouse lines Rosa26/LacZ and Rosa26/YFP and analysis of double transgenic offsprings revealed strong transgene activity in caudal regions of the central nervous system, i.e., brain stem and spinal cord. Some additional Cre expression was observed in cortical and cerebellar regions. In brain stem and spinal cord, Cre expressing cells were identified as glycinergic interneurons by staining with GlyT2- and glycine-immunoreactive antibodies; here, >80% of the glycine-immunoreactive cells expressed the Cre reporter protein. These data indicate that GlyT2-Cre mice are a useful tool for the genetic manipulation of glycinergic interneurons. |
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Authors:
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Naoko Ishihara; Wencke Armsen; Theofilos Papadopoulos; Heinrich Betz; Volker Eulenburg |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genesis (New York, N.Y. : 2000) Volume: 48 ISSN: 1526-968X ISO Abbreviation: Genesis Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-19 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100931242 Medline TA: Genesis Country: United States |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: IM |
Copyright Information:
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(c) 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60529 Frankfurt, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology, metabolism Brain Stem / cytology, metabolism Chromosomes, Artificial, Bacterial / genetics Female Glycine / metabolism* Glycine Plasma Membrane Transport Proteins / genetics* Integrases / genetics* Interneurons / enzymology* Mice Mice, Transgenic* Neurons / cytology, metabolism Pregnancy Promoter Regions, Genetic / genetics Spinal Cord / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycine Plasma Membrane Transport Proteins; 56-40-6/Glycine; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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