Document Detail

Generation of mouse embryonic stem cell lines from zona-free nuclear transfer embryos.
MedLine Citation:
PMID:  20132018     Owner:  NLM     Status:  MEDLINE    
Pluripotent stem cells would have great potential in cell therapies and drug development when genetically matched with the patient; thus, histocompatible cells could be used in transplantation therapy or as a source of patient-specific cells for drug testing. Pluripotent embryonic stem cells (ESCs)-generated via somatic cell nuclear transfer (SCNT) or parthenogenesis (pESC)-are potential sources of histocompatible cells and tissues for transplantation. Earlier studies used the piezoelectric microinjection (PEM) technique for nuclear transfer (NT) in mouse. No specific studies examined zona-free (ZF) NT as an alternative NT method to generate genetically matched ESCs of a nuclear donor. In this study, we compared the efficiency of nuclear transfer-derived ESC (ntESC) line establishment from ZF-NT, ZF-parthenogenetic (PGA), and ZF-fertilized embryos with that of the PEM-NT method. Different nuclei donor cells [cumulus, ESC, and mouse embryonic fibroblast (MEF)] were used and the efficiency of ntESC derivation was investigated, along with their in vitro characterization. The ZF-NT method's efficiency was higher than that of the PEM-NT using cumulus cells. When ESCs and cumulus cells were used as nuclear donor cells, they resulted in significantly higher ZF-NT-derived ntESC line establishment rates compared to MEF cells. In conclusion, the nuclear donor cell type significantly affected the efficiency of ntESC line establishment, and the ZF-NT method was efficient to establish pluripotent ntESC lines.
Julianna Kobolak; Szilard Bodo; Ruttachuk Rungsiwiwut; Qinggang Meng; Marta Adorjan; Pramuan Virutamasen; Mongkol Techakumphu; Andras Dinnyes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular reprogramming     Volume:  12     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-05     Completed Date:  2010-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  105-13     Citation Subset:  IM    
Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, G?d?llo, Hungary.
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MeSH Terms
Cell Line
Cell Nucleus / physiology*
Embryo Transfer / methods*
Embryo, Mammalian / cytology,  physiology
Embryonic Stem Cells / cytology,  physiology*
Mice, Inbred Strains
Nuclear Transfer Techniques / veterinary*
Grant Support
070246//Wellcome Trust

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