| Generation of monoclonal antibodies to recombinant vascular endothelial growth factor. | |
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MedLine Citation:
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PMID: 22808513 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF. |
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Authors:
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S A Shein; O I Gurina; A V Leopol'd; V P Baklaushev; A A Korchagina; N F Grinenko; N V Ivanova; N E Volgina; I A Ryabukhin; V P Chekhonin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bulletin of experimental biology and medicine Volume: 153 ISSN: 1573-8221 ISO Abbreviation: Bull. Exp. Biol. Med. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-07-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372557 Medline TA: Bull Exp Biol Med Country: United States |
Other Details:
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Languages: eng; rus Pagination: 139-42 Citation Subset: IM |
Affiliation:
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Department of Medical Nanobiotechnologies, N. I. Pirogov Russian National Research Medical University; Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health Care and Social Development of the Russian Federation, Moscow, Russia. atomos@rambler.ru. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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