Document Detail


Generation of monoclonal antibodies to recombinant vascular endothelial growth factor.
MedLine Citation:
PMID:  22808513     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.
Authors:
S A Shein; O I Gurina; A V Leopol'd; V P Baklaushev; A A Korchagina; N F Grinenko; N V Ivanova; N E Volgina; I A Ryabukhin; V P Chekhonin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bulletin of experimental biology and medicine     Volume:  153     ISSN:  1573-8221     ISO Abbreviation:  Bull. Exp. Biol. Med.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-07-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372557     Medline TA:  Bull Exp Biol Med     Country:  United States    
Other Details:
Languages:  eng; rus     Pagination:  139-42     Citation Subset:  IM    
Affiliation:
Department of Medical Nanobiotechnologies, N. I. Pirogov Russian National Research Medical University; Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health Care and Social Development of the Russian Federation, Moscow, Russia. atomos@rambler.ru.
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