Document Detail

Generation of monoclonal antibodies to recombinant vascular endothelial growth factor.
MedLine Citation:
PMID:  22808513     Owner:  NLM     Status:  In-Data-Review    
Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.
S A Shein; O I Gurina; A V Leopol'd; V P Baklaushev; A A Korchagina; N F Grinenko; N V Ivanova; N E Volgina; I A Ryabukhin; V P Chekhonin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bulletin of experimental biology and medicine     Volume:  153     ISSN:  1573-8221     ISO Abbreviation:  Bull. Exp. Biol. Med.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-07-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372557     Medline TA:  Bull Exp Biol Med     Country:  United States    
Other Details:
Languages:  eng; rus     Pagination:  139-42     Citation Subset:  IM    
Department of Medical Nanobiotechnologies, N. I. Pirogov Russian National Research Medical University; Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health Care and Social Development of the Russian Federation, Moscow, Russia.
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