| Generation of monoclonal antibodies specific for ORF68 of koi herpesvirus. | |
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MedLine Citation:
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PMID: 21134694 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Outbreaks of koi herpesvirus (KHV) infection in carp are still a serious problem worldwide. KHV is closely related to other two cyprinid herpesviruses, pox herpesvirus (CHV) and haematopoietic necrosis herpesvirus (CyHV-2) in goldfish. In this study, two major KHV antigenic proteins (ORF62 and ORF68) were identified by immunoscreening using a KHV-specific polyclonal antibody, and then monoclonal antibodies were generated for immunodiagnostic studies. After screening hybridoma cells, one mAb against ORF68 (mAb-7C6) was obtained but no mAbs against ORF62. mAb-7C6 specifically reacted with a lysate of KHV-infected koi fin cells (KF-1 cells) but not with lysates of CHV- or CyHV-2-infected KF-1 cells in an immuno-blotting analysis. Similar results were shown in the following tests: (1) a indirect fluorescent antibody test using infected KF-1 cells and (2) an immunohistochemical investigation by fast red stain (infected liver) or FITC detection (infected spleen). These results suggested that mAb-7C6 specifically reacts with KHV ORF68 protein. |
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Authors:
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Takashi Aoki; Tomokazu Takano; Sasimnanas Unajak; Madoka Takagi; Young Rim Kim; Seong Bin Park; Hidehiro Kondo; Ikuo Hirono; Tatsuo Saito-Taki; Jun-Ichi Hikima; Tae Sung Jung |
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Publication Detail:
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Type: Journal Article Date: 2010-12-04 |
Journal Detail:
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Title: Comparative immunology, microbiology and infectious diseases Volume: 34 ISSN: 1878-1667 ISO Abbreviation: Comp. Immunol. Microbiol. Infect. Dis. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7808924 Medline TA: Comp Immunol Microbiol Infect Dis Country: England |
Other Details:
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Languages: eng Pagination: 209-16 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan; Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-710, South Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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