| Generation of monoclonal antibodies against the Galβ1-4Gal epitope: a key tool in studies of species-specific glycans expressed in fish, amphibians, and birds. | |
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MedLine Citation:
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PMID: 22948803 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Whereas the Galβ1-4Gal epitope is rarely found in mammalian glycans, it has been found in glycans of various species of non-mammalian vertebrates, such as fish, amphibians, and birds. Although glycans containing Galβ1-4Gal in these vertebrates were detected by precise structural analysis of the glycans using mass spectrometry and/or NMR spectrometry, there are no convenient methods to detect Galβ1-4Gal from various samples. To analyze systematically the distribution of Galβ1-4Gal in nature, we generated mouse monoclonal antibodies (mAbs) specific for Galβ1-4Gal using extracts of medaka eggs as an immunogen. Four mAbs (two IgMs and two IgG1s) were obtained by ELISA-based screening. The specificities of these mAbs were evaluated by frontal affinity chromatography using 142 kinds of 2-aminopyridine-derivatized oligosaccharides. While all mAbs interacted with (Galβ1-4Gal)-containing oligosaccharides at their non-reducing termini with dissociation constants (K(d)) ranging from 1.0x10(-5) to 2.8x10(-4) M, no apparent interaction was observed with any other glycans. The number of branches containing Galβ1-4Gal on N-glycans did not significantly affect K(d) of mAbs of IgG1-subclasses, but those of IgM-mAbs were decreased by about one order of magnitude, in increments of the number of branches present. Using the mAbs, we established that Galβ1-4Gal is also expressed on glycoproteins in various tissues from the African clawed frog. Immunohistocheminal staining of medaka sections revealed that Galβ1-4Gal epitopes were expressed in the endothelium, epithelium, and epidermis, which directly contact the external environment or invading organisms. Thus, these mAbs are useful for systematically investigating the species-specific expression of glycans, which may act as a barrier against infection. |
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Authors:
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Noriko Suzuki; Daisuke Nawa; Hiroaki Tateno; Takako Yasuda; Shoji Oda; Hiroshi Mitani; Toshiyuki Nishimaki; Takafumi Katsumura; Hiroki Oota; Tsunehiko Hanihara; Atsunori Oga; Jun Hirabayashi; Kazuo Yamamoto |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-3 |
Journal Detail:
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Title: Glycobiology Volume: - ISSN: 1460-2423 ISO Abbreviation: Glycobiology Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9104124 Medline TA: Glycobiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, 277-8562, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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