Document Detail


Generation and live imaging of an endogenous Cdx2 reporter mouse line.
MedLine Citation:
PMID:  22814996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To understand cell fate specification and maintenance during development, it is essential to visualize both lineage markers and cell behaviors in real time using endogenous markers to report cell fate. We have generated a reporter line in which eGFP is fused to the endogenous locus of Cdx2, a transcription factor essential for trophectoderm specification, allowing us to visualize cell fate decisions in the preimplantation mouse embryo. We used two-photon laser scanning microscopy to visualize expression of the endogenous Cdx2 fusion protein and show that Cdx2 undergoes phases of upregulation. Additionally, we show that as late as the 32-cell stage, outer trophectoderm cells may change their fates by migrating inward and losing Cdx2 expression. Furthermore, the tools and techniques we report allow for dual-colored imaging, which will greatly facilitate the study of not only preimplantation development, but later stages of development and tissues where Cdx2 plays an important role.
Authors:
Katie McDole; Yixian Zheng
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-16
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  50     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-19     Completed Date:  2013-03-20     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  775-82     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA. mcdole@ciwemb.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology
Cell Lineage
Embryo, Mammalian / metabolism
Founder Effect
Genes, Reporter
Green Fluorescent Proteins / genetics,  metabolism
Homeodomain Proteins / genetics*,  metabolism
Mice
Mice, Transgenic
Microscopy, Fluorescence
Recombinant Proteins
Transcription Factors / genetics*,  metabolism
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
R21 AA020060/AA/NIAAA NIH HHS; R21 AA020060-02/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/Cdx2 protein, mouse; 0/Homeodomain Proteins; 0/Recombinant Proteins; 0/Transcription Factors; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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