| Generation and live imaging of an endogenous Cdx2 reporter mouse line. | |
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MedLine Citation:
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PMID: 22814996 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To understand cell fate specification and maintenance during development, it is essential to visualize both lineage markers and cell behaviors in real time using endogenous markers to report cell fate. We have generated a reporter line in which eGFP is fused to the endogenous locus of Cdx2, a transcription factor essential for trophectoderm specification, allowing us to visualize cell fate decisions in the preimplantation mouse embryo. We used two-photon laser scanning microscopy to visualize expression of the endogenous Cdx2 fusion protein and show that Cdx2 undergoes phases of upregulation. Additionally, we show that as late as the 32-cell stage, outer trophectoderm cells may change their fates by migrating inward and losing Cdx2 expression. Furthermore, the tools and techniques we report allow for dual-colored imaging, which will greatly facilitate the study of not only preimplantation development, but later stages of development and tissues where Cdx2 plays an important role. |
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Authors:
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Katie McDole; Yixian Zheng |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-08-16 |
Journal Detail:
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Title: Genesis (New York, N.Y. : 2000) Volume: 50 ISSN: 1526-968X ISO Abbreviation: Genesis Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-19 Completed Date: 2013-03-20 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 100931242 Medline TA: Genesis Country: United States |
Other Details:
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Languages: eng Pagination: 775-82 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA. mcdole@ciwemb.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology Cell Lineage Embryo, Mammalian / metabolism Founder Effect Genes, Reporter Green Fluorescent Proteins / genetics, metabolism Homeodomain Proteins / genetics*, metabolism Mice Mice, Transgenic Microscopy, Fluorescence Recombinant Proteins Transcription Factors / genetics*, metabolism Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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R21 AA020060/AA/NIAAA NIH HHS; R21 AA020060-02/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cdx2 protein, mouse; 0/Homeodomain Proteins; 0/Recombinant Proteins; 0/Transcription Factors; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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