Document Detail


Generation of leukotriene B4, its all trans isomers and 5-hydroxyeicosatetraenoic acid by rat basophilic leukaemia cells.
MedLine Citation:
PMID:  6282374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1 When rat basophilic leukaemia (RBL-1) cells were incubated for 4 min, in the presence of arachidonic acid (AA) and calcium ionophore A23187, three isomers of leukotriene (LT) B4 and 5-hydroxyeicosatetraenoic acid (5-HETE) were generated. 2 The isomers of LTB4 separated by reverse phase high pressure liquid chromatography (h.p.l.c.) were found to be 5 (s)-dihydroxy-6, 14-trans-8,10-trans-eicosatetraenoic acid: 5(S), 12(R)-dihydroxy-6,14-trans-8,10-trans-eicosatetraenoic acid and 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid or LTB4. 3 5(S), 12(R)-cis, trans, trans-LTB4 produced by RBL-1 cells caused aggregation of rat polymorphonuclear leucocytes (PMNs). Its other two isomers (5(S), 12(S)-all trans-LTB4; 5(S), 12-(R)-all trans-LTB4) were much less active and 5-HETE was found to be inactive. 4 5(S), 12(R)-cis, trans, trans-LTB4 formed from RBL-1 cells contracted guinea-pig parenchymal strips (GPPs) and was indistinguishable from synthetic 5(S), 12(R)-cis, trans, trans-LTB4. The other two isomers of LTB4. i.e. 5(S), 12(S)-all trans-LTB4 and 5(S), 12(R)-all trans-LTB4 as well as 5-HETE were inactive on GPP. 5 RBL-1 LTB4 was equipotent with LTD4. in most experiments, in contracting GPP but had no activity on guinea-pig ileum smooth muscle (GPISM). 6 RBL-1 cells contain the enzyme systems necessary for generating LTB4 as well as the leukotriene(s) with amino acid residues at C-6.
Authors:
A W Ford-Hutchinson; P J Piper; M N Samhoun
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  76     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-08-07     Completed Date:  1982-08-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  215-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acids / metabolism*
Basophils / metabolism
Cell Aggregation / drug effects
Cells, Cultured
Guinea Pigs
Hydroxyeicosatetraenoic Acids*
Leukemia, Experimental / metabolism*
Leukotriene B4
Rats
Rats, Inbred Strains
Stereoisomerism
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Hydroxyeicosatetraenoic Acids; 71160-24-2/Leukotriene B4; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid
Comments/Corrections

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