Document Detail


Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices.
MedLine Citation:
PMID:  15100775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The high speed production of fluid segments for the highly parallelized cultivation of monoclonal cell populations was carried out by the use of microchip segmentor modules. Aqueous fluid segments, embedded in a non-miscible carrier liquid, were produced with frequencies up to 30 s(-1) and showed a high homogeneity in size. This corresponds with the production of about 2.5 million samples per day. The segment volumes can be adapted between about 4 nl and 100 nl. The typical segment size for cultivation experiments is in the range between 40 nl and 80 nl. Nutrient medium can be applied instead of pure water. It is possible to aliquot a cell suspension in such a way that most of the aqueous fluid segments contain only one cell. In model experiments with four microbial species chip-produced aliquots of 60 nl, each containing one or a few cells, were incubated in Teflon capillary tubes. Rapid growth of the microcultures was observed. Cell densities were found to be as high as in conventional shake flask cultures.
Authors:
Karin Martin; Thomas Henkel; Volker Baier; Andreas Grodrian; Thore Schön; Martin Roth; Johann Michael Köhler; Josef Metze
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-06-03
Journal Detail:
Title:  Lab on a chip     Volume:  3     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2004-04-21     Completed Date:  2004-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  202-7     Citation Subset:  IM    
Affiliation:
Hans Knöll Institute for Natural Products Research, Jena, Germany.
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Culture Media
Escherichia coli / genetics,  growth & development
Fluorescence
Microbiological Techniques / instrumentation*,  methods*
Microfluidics / instrumentation*
Polytetrafluoroethylene
Pseudomonas fluorescens / genetics,  growth & development
Rhodococcus / genetics,  growth & development
Saccharomyces cerevisiae / genetics,  growth & development
Time Factors
Chemical
Reg. No./Substance:
0/Culture Media; 9002-84-0/Polytetrafluoroethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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