Document Detail

Generation of labeled probes by PCR.
MedLine Citation:
PMID:  21390779     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Nucleic acid probes are an important tool in molecular diagnosis. To facilitate the detection of hybridized probes, they are labeled with a reporter molecule, which is usually a radioisotope. For diagnostic techniques carried out in a clinical laboratory, radioisotopes are hazardous and, thus, recently there is a move to use nonisotopic labels, such as biotin and digoxigenin. Nonisotopic probes also have the advantage of much better stability over time compared with isotopic probes that have limited half-lives.
Y M Lo; S F An
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  16     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  1998  
Date Detail:
Created Date:  2011-03-10     Completed Date:  2012-10-02     Revised Date:  2014-07-29    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  93-100     Citation Subset:  -    
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