Document Detail

Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system.
MedLine Citation:
PMID:  10749735     Owner:  NLM     Status:  MEDLINE    
This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pH(i)) within single ventricular myocytes. The spatial distribution of pH(i) in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 microm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pH(i) gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should be useful for studying the effects of localized acidosis on myocyte function, estimating intracellular ion diffusion rates, and, possibly, inducing regional changes in other important intracellular ions.
K W Spitzer; P R Ershler; R L Skolnick; R D Vaughan-Jones
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  278     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-04-27     Completed Date:  2000-04-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1371-82     Citation Subset:  IM    
Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, Utah 84112, USA.
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MeSH Terms
Acidosis / metabolism*
Ammonium Chloride / pharmacology
Carbon Dioxide / pharmacology
Cells, Cultured
Diffusion Chambers, Culture / instrumentation,  methods
HEPES / pharmacology
Heart Ventricles / cytology,  metabolism
Hydrogen-Ion Concentration*
Microscopy, Confocal
Muscle Fibers, Skeletal / drug effects,  metabolism*
Myocardium / cytology,  metabolism*
Propionic Acids / pharmacology
Sarcolemma / drug effects,  metabolism
Grant Support
Reg. No./Substance:
0/Buffers; 0/Propionic Acids; 0/sodium propionate; 12125-02-9/Ammonium Chloride; 124-38-9/Carbon Dioxide; 7365-45-9/HEPES; 79-09-4/propionic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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