Document Detail


Generation of human induced pluripotent stem cells from oral mucosa.
MedLine Citation:
PMID:  20547351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Induced pluripotent stem (iPS) cells are one of the most promising sources for cell therapy in regenerative medicine. Using a patient's own genetically identical and histocompatible cells is the ideal way to practice personalized regenerative medicine. For personalized iPS cell therapy, the prerequisites for cell source preparation are a simple and safe procedure, no aesthetic or functional damage, and quick wound healing. Oral mucosa fibroblasts (OFs) may have high potential to fulfill these requirements. In this study, biopsy was performed in a dental chair; no significant incisional damage was recognized and rapid wound healing (within a week) was observed. We generated human iPS cells from the isolated OFs via the retroviral gene transfer of OCT4, SOX2, c-MYC, and KLF4. Reprogrammed cells showed ES-like morphology and expressed undifferentiated markers such as OCT4, NANOG, SSEA4, TRA-1-60, and TRA-1-81. Subsequent in vitro and in vivo analyses confirmed the pluripotency of resultant iPS cells, which matched the criteria for iPS cells. In addition, we found that the endogenous expression levels of c-MYC and KLF4 in OFs were similar to those in dermal fibroblasts. Taken together, we propose that OFs could be a practical source for preparing iPS cells to achieve personalized regenerative medicine in the near future.
Authors:
Keiko Miyoshi; Daisuke Tsuji; Keiko Kudoh; Kazuhito Satomura; Taro Muto; Kohji Itoh; Takafumi Noma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-08
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  110     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  345-50     Citation Subset:  IM    
Copyright Information:
Copyright 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Cell Culture Techniques / methods*
Cell Differentiation
Female
Genetic Enhancement / methods*
Humans
Male
Mouth Mucosa / cytology*,  physiology*
Pluripotent Stem Cells / cytology*,  physiology*
Tissue Engineering / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Generation of reactive oxygen species from conventional laboratory media.
Next Document:  Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasi...