Document Detail


Generation of a germ cell nuclear factor conditional allele in mice.
MedLine Citation:
PMID:  14666510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two recombination steps in embryonic stem (ES) cells were adopted to generate a floxed Germ Cell Nuclear Factor (GCNF) allele. First, a targeting vector containing a loxP site upstream of exon 4, encoding the DNA binding domain (DBD), and a floxed NeoTK double selection cassette downstream of exon 4 was integrated into the GCNF locus by homologous recombination. Second, a Cre-expressing vector was transiently introduced to remove the floxed NeoTK cassette via site-specific recombination. Heterogeneous ES cell populations were found in a single colony after Cre transfection and were separated using an ES cell re-pick step. Floxed GCNF mice were generated and had normal GCNF expression in the adult gonads. Using the Msx2Cre transgenic mice, the floxed GCNF can be completely deleted in the female germline. Taken together, the floxed GCNF mice were successfully generated and female germline deletion of the floxed GCNF allele was achieved using Msx2Cre mice.
Authors:
Zi-Jian Lan; Xueping Xu; Austin J Cooney
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  37     ISSN:  1526-954X     ISO Abbreviation:  Genesis     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-10     Completed Date:  2004-08-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  172-9     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Affiliation:
Department of Molecular and Cellular Biology, Baylor College of Medicine, BCMM-M733, Houston, Texas 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
DNA-Binding Proteins / genetics*
Embryo, Mammalian / cytology
Exons
Gene Targeting
Genetic Vectors
Gonads / cytology,  metabolism*
Integrases
Mice
Mice, Transgenic
Nuclear Receptor Subfamily 6, Group A, Member 1
Receptors, Cytoplasmic and Nuclear / genetics*
Recombination, Genetic*
Stem Cells / metabolism
Viral Proteins
Grant Support
ID/Acronym/Agency:
HD07495/HD/NICHD NIH HHS; HD32878/HD/NICHD NIH HHS; T32 DK07763/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Nr6a1 protein, mouse; 0/Nuclear Receptor Subfamily 6, Group A, Member 1; 0/Receptors, Cytoplasmic and Nuclear; 0/Viral Proteins; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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