| Generation of free radicals during lipid hydroperoxide-triggered apoptosis in PC12h cells. | |
| | |
MedLine Citation:
|
PMID: 9084499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The compound 13-L-hydroperoxylinoleic acid (LOOH) triggered the death of clonal rat pheochromocytoma PC12h cells (LD50 = about 8 microM). LOOH induced nuclear condensation and DNA fragmentation, which was prevented by cycloheximide (a protein synthesis inhibitor) and NGF, indicating that LOOH triggered apoptosis in PC12h cells. LOOH produced reactive oxygen species (ROS) in PC12h cells in a time- and dose-dependent manner, as measured by flow cytometry using the ROS-specific fluorescent indicator, 6-carboxy-2,7-dichorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA). Antioxidants such as N,N'-diphenyl-p-phenylenediamine (DPPD), vitamin E and N-acetylcysteine, and a ferric iron chelator, deferoxamine, inhibited the LOOH-triggered apoptosis and simultaneously decreased the generation of ROS, whereas an inhibitor of glutathione synthesis, buthionine sulfoximine (BSO), enhanced the apoptosis and increased the generation of ROS. These results indicate that LOOH triggers the apoptosis of PC12h cells by increasing the production of ROS. A confocal analysis with the Ca(2+)-specific fluorescent indicator, fluo-3, demonstrated that LOOH at concentrations up to 200 microM, did not increase the intracellular Ca2+ concentration. These data indicate that LOOH induces apoptosis of PC12h cells through the enhanced production of ROS, not through increasing the permeability of Ca2+. |
| | |
Authors:
|
H Aoshima; T Satoh; N Sakai; M Yamada; Y Enokido; T Ikeuchi; H Hatanaka |
Related Documents
:
|
1620729 - Quenching of singlet oxygen by biomolecules from l1210 leukemia cells. 18001129 - Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-res... 11717389 - The human salivary peptide histatin 5 exerts its antifungal activity through the format... 17394299 - Highly photocytotoxic glucosylated silicon(iv) phthalocyanines. effects of peripheral c... 6718689 - The effect on the km for radiosensitization at 0 degree c of thiol depletion by diethyl... 7378899 - Quantitative correlation between the reduction of nitroblue tetrazolium and the oxygen ... 2056349 - The use of confocal microscopy and sterecon reconstructions in the analysis of sea urch... 20204289 - Up-regulated expression of stathmin may be associated with hepatocarcinogenesis. 11691789 - Polyamine depletion in human melanoma cells leads to g1 arrest associated with inductio... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1345 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1997 Mar |
Date Detail:
|
Created Date: 1997-04-09 Completed Date: 1997-04-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 35-42 Citation Subset: IM |
Affiliation:
|
Division of Protein Biosynthesis, Osaka University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis* Calcium / analysis Cell Survival DNA Fragmentation Flow Cytometry Free Radicals / chemical synthesis* Linoleic Acids / pharmacology* PC12 Cells / drug effects Rats Reactive Oxygen Species* |
| Chemical | |
Reg. No./Substance:
|
0/Free Radicals; 0/Linoleic Acids; 0/Reactive Oxygen Species; 34444-18-3/13-hydroperoxylinoleic acid; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Formation of leukotoxin (9,10-epoxy-12-octadecenoic acid) during the autoxidation of phospholipids p...
Next Document: Serum-liposome interaction is an oxygen-dependent process.