| Generation of a doxycycline-inducible KSHV producer cell line of endothelial origin: maintenance of tight latency with efficient reactivation upon induction. | |
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MedLine Citation:
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PMID: 21419799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma (KS) and at least two B cell lymphoproliferative diseases: primary effusion lymphoma (PEL) and multicentric Castleman's disease (MCD). B cells derived from PEL are latently infected, and can be induced to lytic replication by treatment with chemical agents like TPA or butyrate, which have pleiotropic effects on host cell signaling and chromatin structure. Most of these lines also display moderate levels of spontaneous lytic induction, which complicates analysis of latency. Here we describe the creation of latently infected cell lines derived from SLK endothelial cells that (i) display tight control of KSHV latency, with little spontaneous reactivation and (ii) are efficiently inducible by doxycycline, avoiding the need for pleiotropic inducing agents. These cells produce substantial quantities of infectious KSHV, and should be useful for studies of the latent-lytic switch and the impact of lytic replication on host cell biology. |
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Authors:
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Jinjong Myoung; Don Ganem |
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Publication Detail:
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Type: Journal Article Date: 2011-03-17 |
Journal Detail:
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Title: Journal of virological methods Volume: 174 ISSN: 1879-0984 ISO Abbreviation: J. Virol. Methods Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-16 Completed Date: 2011-08-23 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 8005839 Medline TA: J Virol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 12-21 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Howard Hughes Medical Institute, Departments of Microbiology & Medicine and GW Hooper Foundation, University of California, San Francisco, San Francisco, CA 94143, USA. jinjong.myoung@ucsf.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Doxycycline / metabolism* Gene Expression Regulation / drug effects* Herpesvirus 8, Human / genetics, growth & development* Humans Virus Activation / drug effects* Virus Cultivation / methods Virus Latency / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA096491-05/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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564-25-0/Doxycycline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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