Document Detail


Generation of a doxycycline-inducible KSHV producer cell line of endothelial origin: maintenance of tight latency with efficient reactivation upon induction.
MedLine Citation:
PMID:  21419799     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma (KS) and at least two B cell lymphoproliferative diseases: primary effusion lymphoma (PEL) and multicentric Castleman's disease (MCD). B cells derived from PEL are latently infected, and can be induced to lytic replication by treatment with chemical agents like TPA or butyrate, which have pleiotropic effects on host cell signaling and chromatin structure. Most of these lines also display moderate levels of spontaneous lytic induction, which complicates analysis of latency. Here we describe the creation of latently infected cell lines derived from SLK endothelial cells that (i) display tight control of KSHV latency, with little spontaneous reactivation and (ii) are efficiently inducible by doxycycline, avoiding the need for pleiotropic inducing agents. These cells produce substantial quantities of infectious KSHV, and should be useful for studies of the latent-lytic switch and the impact of lytic replication on host cell biology.
Authors:
Jinjong Myoung; Don Ganem
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Publication Detail:
Type:  Journal Article     Date:  2011-03-17
Journal Detail:
Title:  Journal of virological methods     Volume:  174     ISSN:  1879-0984     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-16     Completed Date:  2011-08-23     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  12-21     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Doxycycline / metabolism*
Gene Expression Regulation / drug effects*
Herpesvirus 8, Human / genetics,  growth & development*
Humans
Virus Activation / drug effects*
Virus Cultivation / methods
Virus Latency / drug effects*
Grant Support
ID/Acronym/Agency:
R01 CA096491/CA/NCI NIH HHS; R01 CA096491-05/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
N12000U13O/Doxycycline
Comments/Corrections

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