| Generation of a cholesterol-independent, non-GS NS0 cell line through chemical treatment and application for high titer antibody production. | |
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MedLine Citation:
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PMID: 22252532 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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NS0 cells require exogenous cholesterol for growth. The non-GS cholesterol-dependent NS0 host was treated with 5-azacytidine (5azaC), a demethylation drug, and adapted to grow in cholesterol-free, chemically-defined medium. Within seven weeks, a stable, cholesterol-independent NS0 host (NS0.CF) was obtained. The new NS0.CF host, as well as the original cholesterol auxotroph host, was transfected with the same mAb expression plasmid, and the top producing clone from both hosts were compared side-by-side in the enhanced platform fed-batch cultures using chemically-defined media. The NS0.CF derived clone significantly out-performed the cholesterol-dependent clone, with titer reaching 4.5 g/L vs 3.0 g/L, respectively, mainly due to higher specific productivity, while key product quality attributes remained comparable. This work demonstrated an effective and rapid approach to generate a cholesterol-independent NS0 host, and its application in recombinant protein production. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc. |
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Authors:
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Jincai Li; Wei Gu; Diane G Edmondson; Connie Lu; Nattu Vijayasankaran; Bruno Figueroa; Dave Stevenson; Thomas Ryll; Feng Li |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-17 |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: - ISSN: 1097-0290 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Oceanside Pharma Technical Development, Genentech, Inc, Oceanside, CA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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