| Generation and characterization of a rat monoclonal antibody specific for PCNA. | |
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MedLine Citation:
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PMID: 18642673 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This essential cellular component, originally characterized as a nuclear antigen of dividing cells, is evolutionary highly conserved from yeast to human. Within the eukaryotic cell, PCNA serves as a processivity factor for DNA polymerase delta and plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodelling. All these cellular processes are regulated by a complex network comprising cell cycle dependent changes in expression levels, dynamics, interactions, and localization of PCNA. Here we report the generation and characterization of the first rat monoclonal antibody (MAb) against human PCNA, designated as PCNA 16D10. We demonstrated that PCNA 16D10 MAb has high affinity and specificity and is suited for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence. The characteristic punctate staining of S phase cells allows the identification of proliferating cells and the monitoring of cell cycle progression. |
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Authors:
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Andrea Rottach; Elisabeth Kremmer; Danny Nowak; Prisca Boisguerin; Rudolf Volkmer; M Cristina Cardoso; Heinrich Leonhardt; Ulrich Rothbauer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hybridoma (2005) Volume: 27 ISSN: 1554-0014 ISO Abbreviation: Hybridoma (Larchmt) Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-07-22 Completed Date: 2008-08-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101241539 Medline TA: Hybridoma (Larchmt) Country: United States |
Other Details:
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Languages: eng Pagination: 91-8 Citation Subset: IM |
Affiliation:
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Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Antinuclear / biosynthesis, chemistry*, diagnostic use Antibodies, Monoclonal / biosynthesis*, chemistry*, diagnostic use Cell Line, Tumor Cricetinae Enzyme-Linked Immunosorbent Assay Epitope Mapping Hela Cells Humans Immunoblotting Mice Molecular Sequence Data Proliferating Cell Nuclear Antigen / genetics, immunology* Rats |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Antinuclear; 0/Antibodies, Monoclonal; 0/Proliferating Cell Nuclear Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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