| Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene. | |
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MedLine Citation:
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PMID: 20049953 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CLIC1 belongs to a family of highly conserved and widely expressed intracellular chloride ion channel proteins existing in both soluble and membrane integrated forms. To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1(FN)) allele, followed by Clic1 knock-out (Clic1(-/-)) mice by crossing Clic1(FN) allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal. However, Clic1(-) (/-) mice show a mild platelet dysfunction characterized by prolonged bleeding times and decreased platelet activation in response to adenosine diphosphate stimulation linked to P2Y(12) receptor signaling. |
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Authors:
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Min Ru Qiu; Lele Jiang; Klaus I Matthaei; Simone M Schoenwaelder; Tamara Kuffner; Pierre Mangin; Joanne E Joseph; Joyce Low; David Connor; Stella M Valenzuela; Paul M G Curmi; Louise J Brown; Martyn Mahaut-Smith; Shaun P Jackson; Samuel N Breit |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Genesis (New York, N.Y. : 2000) Volume: 48 ISSN: 1526-968X ISO Abbreviation: Genesis Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-09 Completed Date: 2010-04-27 Revised Date: 2010-05-26 |
Medline Journal Info:
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Nlm Unique ID: 100931242 Medline TA: Genesis Country: United States |
Other Details:
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Languages: eng Pagination: 127-36 Citation Subset: IM |
Copyright Information:
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(c) 2010 Wiley-Liss, Inc. |
Affiliation:
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St. Vincent's Centre for Applied Medical Research, St. Vincent's Hospital and University of New South Wales, Sydney, New South Wales, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Animals Blood Platelets / metabolism Chloride Channels / genetics* Crosses, Genetic Gene Deletion* Gene Targeting / methods* Genetic Engineering* Hemorrhage Heterozygote Homozygote Immunohistochemistry Integrases / metabolism Mice Mice, Knockout Models, Genetic* Recombination, Genetic |
| Chemical | |
Reg. No./Substance:
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0/CLC-1 channel; 0/Chloride Channels; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases |
| Comments/Corrections | |
Erratum In:
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Genesis. 2010 May;48(5):351 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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