Document Detail


Generation and characterization of a large panel of murine monoclonal antibodies against vaccinia virus.
MedLine Citation:
PMID:  21056889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vaccinia virus (VACV), the vaccine for smallpox, induces an antibody response that is largely responsible for conferring protection. Here, we studied the antibody response to VACV by generating and characterizing B cell hybridomas from a mouse immunized with VACV. Antibodies from 66 hybridomas were found to recognize 11 VACV antigens (D8, A14, WR148, D13, H3, A56, A33, C3, B5, A10 and F13), 10 of which were previously recognized as major antigens in smallpox vaccine by a microarray of VACV proteins produced with a prokaryotic expression system. VACV C3 protein, which was not detected as a target of antibody response by the proteome array, was recognized by two hybridomas, suggesting that selection of hybridomas based on immune recognition of infected cells has the advantage of detecting additional antibody response to native VACV antigens. In addition, these monoclonal antibodies are valuable reagents for studying poxvirus biology and protective mechanism of smallpox vaccine.
Authors:
Xiangzhi Meng; Youmin Zhong; Addie Embry; Bo Yan; Shan Lu; Guangming Zhong; Yan Xiang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-11-05
Journal Detail:
Title:  Virology     Volume:  409     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-21     Completed Date:  2011-01-18     Revised Date:  2014-11-09    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  271-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*,  isolation & purification*
Antibodies, Viral / immunology*,  isolation & purification*
Antigens, Viral / immunology
Mice
Mice, Inbred BALB C
Protein Array Analysis
Vaccinia virus / immunology*
Grant Support
ID/Acronym/Agency:
AI064537/AI/NIAID NIH HHS; AI079217/AI/NIAID NIH HHS; R01 AI064537/AI/NIAID NIH HHS; R01 AI064537-01/AI/NIAID NIH HHS; R01 AI079217/AI/NIAID NIH HHS; R01 AI079217-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Antigens, Viral
Comments/Corrections

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