| Generation and characterization of human monoclonal neutralizing antibodies with distinct binding and sequence features against SARS coronavirus using XenoMouse. | |
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MedLine Citation:
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PMID: 17161858 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Passive therapy with neutralizing human monoclonal antibodies (mAbs) could be an effective therapy against severe acute respiratory syndrome coronavirus (SARS-CoV). Utilizing the human immunoglobulin transgenic mouse, XenoMouse, we produced fully human SARS-CoV spike (S) protein specific antibodies. Antibodies were examined for reactivity against a recombinant S1 protein, to which 200 antibodies reacted. Twenty-seven antibodies neutralized 200TCID(50) SARS-CoV (Urbani). Additionally, 57 neutralizing antibodies were found that are likely specific to S2. Mapping of the binding region was achieved with several S1 recombinant proteins. Most S1 reactive neutralizing mAbs bound to the RBD, aa 318-510. However, two S1 specific mAbs reacted with a domain upstream of the RBD between aa 12 and 261. Immunoglobulin gene sequence analyses suggested at least 8 different binding specificities. Unique human mAbs could be used as a cocktail that would simultaneously target several neutralizing epitopes and prevent emergence of escape mutants. |
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Authors:
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Melissa Coughlin; Gin Lou; Osvaldo Martinez; Stephanie K Masterman; Ole A Olsen; Angelica A Moksa; Michael Farzan; John S Babcook; Bellur S Prabhakar |
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Publication Detail:
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Type: Journal Article Date: 2006-12-11 |
Journal Detail:
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Title: Virology Volume: 361 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-16 Completed Date: 2007-06-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 93-102 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology (MC790) College of Medicine, University of Illinois at Chicago, Room E705. 835 S. Wolcott AveChicago, IL 60612, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Monoclonal / biosynthesis, genetics, immunology* Antibodies, Viral / biosynthesis, genetics, immunology* Antibody Specificity Hemagglutinins, Viral / immunology Humans Hybridomas Immunoglobulins / genetics, metabolism Membrane Glycoproteins / immunology Mice Mice, Transgenic Molecular Sequence Data Neutralization Tests SARS Virus / immunology* Sequence Alignment Severe Acute Respiratory Syndrome Viral Envelope Proteins / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Hemagglutinins, Viral; 0/Immunoglobulins; 0/Membrane Glycoproteins; 0/Viral Envelope Proteins; 107476-75-5/spike glycoprotein, coronavirus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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