Document Detail


Generation and characterization of KsprtTA and KsptTA transgenic mice.
MedLine Citation:
PMID:  23420736     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The advent of technologies that allow tissue specific expression or ablation of genes has contributed enormously to our knowledge of the mechanism regulating organ development and maintenance in mice. The tetracycline inducible system allows reversible regulation of gene products upon administration of doxycycline. Here we describe the generation and activity of two transgenic lines expressing the cDNAs for the Tet responsive transcription factors rtTA and tTA (Tet-on and off) respectively under the control of an element that drives expression in the epithelium of the developing and adult kidney. Both lines show inducible and reversible activity in the embryonic and adult organ.
Authors:
Xinchao Pan; Erin V Small; Peter Igarashi; Thomas J Carroll
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-28
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  51     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-27     Completed Date:  2014-01-14     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  430-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadherins / genetics*,  metabolism
Doxycycline / pharmacology
Epithelial Cells / cytology,  metabolism*
Female
Gene Expression Regulation, Developmental*
Integrases / genetics,  metabolism
Kidney / cytology,  embryology*,  metabolism*
Mice
Mice, Transgenic*
Nephrons / metabolism*
Organ Culture Techniques
Pregnancy
Promoter Regions, Genetic
Transcriptional Activation
Grant Support
ID/Acronym/Agency:
P30 DK079328/DK/NIDDK NIH HHS; P30DK079328/DK/NIDDK NIH HHS; R01 DK080004/DK/NIDDK NIH HHS; R01 DK095057/DK/NIDDK NIH HHS; R01DK080004/DK/NIDDK NIH HHS; R01DK095057/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins; 0/Cdh16 protein, mouse; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases; N12000U13O/Doxycycline
Comments/Corrections

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