| Generation of cell lines to study the role played by oncogenes and anti-oncogenes in cell proliferation control. | |
| | |
MedLine Citation:
|
PMID: 2101319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We report the generation of stable transfectant cell lines by DNA-mediated transfection that overexpress viral and/or cellular oncogenes. Expression of heterologous genes (FBJ- and FBR-v-fos, polyoma large and middle T) was confirmed by Northern hybridization, immunofluorescence and immunoprecipitation. We also describe the isolation of two retinoblastoma cell lines from human tumors. Neuronal and glial markers were used to confirm the origin of these cell lines. Oncogene transfectant and retinoblastoma cell lines will be used to assess Rb expression and the possible role of its gene product in cell proliferation control and neoplasia. |
| | |
Authors:
|
E Costanzi; L E Rameh; C A Joazeiro; M C Armelin |
Related Documents
:
|
20416459 - In vitro expression and characterization of myh9 mutant alleles linked to hereditary he... 15184049 - Selective depletion of the type i, type ii, and type iii isozymes of hexokinase in mamm... 19771229 - Potent subunit-specific effects on cell growth and drug sensitivity from optimised sirn... 10075999 - Long-term stability of large insert genomic dna episomal shuttle vectors in human cells. 19504139 - Plasmid stability and kinetics of continuous production of glucoamylase by recombinant ... 17309779 - The upregulation of expressed proteins in hepg2 cells transfected by the recombinant pl... 18286619 - Boronate-containing polymer brushes: characterization, interaction with saccharides and... 11231069 - Misexpression of igf-i in the mouse lens expands the transitional zone and perturbs len... 22163449 - Application of sensing techniques to cellular force measurement. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] Volume: 23 ISSN: 0100-879X ISO Abbreviation: Braz. J. Med. Biol. Res. Publication Date: 1990 |
Date Detail:
|
Created Date: 1991-09-06 Completed Date: 1991-09-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8112917 Medline TA: Braz J Med Biol Res Country: BRAZIL |
Other Details:
|
Languages: eng Pagination: 795-9 Citation Subset: IM |
Affiliation:
|
Departamento de Bioquímica, Universidade de São Paulo, Brasil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Division
/
physiology Cell Line, Transformed Gene Expression Regulation, Neoplastic / physiology* Genes, Tumor Suppressor / physiology* Humans Oncogenes / physiology* Phenotype Retinoblastoma / genetics Transfection |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Binding of the anti-inflammatory drug niflumic acid to bovine serum albumin.
Next Document: The use of a new non-verbal test in the evaluation of recent memory.