Document Detail

Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes.
MedLine Citation:
PMID:  18576148     Owner:  NLM     Status:  MEDLINE    
Prion-propagating cell lines are an efficient and useful means for studying the cellular and molecular mechanisms implicated in prion disease. Use of cell-based models has lead to the finding that prion protein (PrP(C)) and PrP(Sc) are released from cells in association with exosomes. Furthermore, exosomes have been shown to act as vehicles for infectivity, transferring PrP(Sc) between cell lines and providing a mechanism for prion spread between tissues. As a role for exosomes in prion disease is emerging, this chapter outlines a method for the generation of prion-infected cell lines and the isolation and characterization of PrP(C)- and PrP(Sc)-containing exosomes.
Laura J Vella; Andrew F Hill
Related Documents :
10738898 - Fluorescence spectroscopy of normal, sv40-transformed human keratinocytes, and carcinom...
20231018 - Identification and characterization of autoantibody-producing b220(low) b (b-1) cells a...
19675188 - Establishment of an insect cell clone that harbours a partial baculoviral genome and is...
7989438 - Survey of cell lines in the american type culture collection for bovine viral diarrhea ...
21738728 - Tspyl2 is important for g1 checkpoint maintenance upon dna damage.
18621168 - Distribution of sulfhydryloxidase (sox) immunoreactivity in the testis of the axolotl (...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  459     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-06-25     Completed Date:  2008-08-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  69-82     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Melbourne, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Assay
Cell Culture Techniques / methods*
Cell Line
PrPSc Proteins / metabolism
Prion Diseases
Prions / biosynthesis*,  pathogenicity*
Secretory Vesicles / metabolism*
Reg. No./Substance:
0/PrPSc Proteins; 0/Prions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Assaying prions in cell culture: the standard scrapie cell assay (SSCA) and the scrapie cell assay i...
Next Document:  Neurotoxicity of prion peptides on cultured cerebellar neurons.