Document Detail


Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes.
MedLine Citation:
PMID:  18576148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prion-propagating cell lines are an efficient and useful means for studying the cellular and molecular mechanisms implicated in prion disease. Use of cell-based models has lead to the finding that prion protein (PrP(C)) and PrP(Sc) are released from cells in association with exosomes. Furthermore, exosomes have been shown to act as vehicles for infectivity, transferring PrP(Sc) between cell lines and providing a mechanism for prion spread between tissues. As a role for exosomes in prion disease is emerging, this chapter outlines a method for the generation of prion-infected cell lines and the isolation and characterization of PrP(C)- and PrP(Sc)-containing exosomes.
Authors:
Laura J Vella; Andrew F Hill
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  459     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-06-25     Completed Date:  2008-08-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  69-82     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Melbourne, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay
Cell Culture Techniques / methods*
Cell Line
Exocytosis*
Mice
PrPSc Proteins / metabolism
Prion Diseases
Prions / biosynthesis*,  pathogenicity*
Secretory Vesicles / metabolism*
Chemical
Reg. No./Substance:
0/PrPSc Proteins; 0/Prions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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