Document Detail

Generation of an antibody specific to erythritol, a non-immunogenic food additive.
MedLine Citation:
PMID:  16901854     Owner:  NLM     Status:  MEDLINE    
Erythritol, a simple sugar alcohol, is widely used as a food and drug additive owing to its chemical inertness, sweetness and non-toxicity. Adverse reactions to erythritol are rare and only three cases of allergic reactions to foods containing erythritol have been reported. Being inert, erythritol cannot produce an immunological response. In order to explain the mechanism of immunogenicity of erythritol, a method to obtain erythritol epitopes on a carrier protein, which can serve as an immunogen to develop antibodies against erythritol, is described. D-Erythrose was conjugated to bovine serum albumin at pH 8 by reductive amination. The reduction product of the Schiff base of D-erythrose-bovine serum albumin conjugate creates erythritoyl groups. Rabbits immunized with erythritol-bovine serum albumin conjugate (29 haptens/molecule) showed good antibody response (detection of 1 microg antigen, erythritol-keyhole limpet haemocyanin conjugate possessing 50% modified amino groups, at 1 : 50,000 dilution). Anti-erythritol immunoglobulin-G antibodies were purified from the immune serum using hapten-affinity chromatography on an erythritol-keyhole limpet haemocyanin-Sepharose CL-6B affinity matrix. The yield of erythritol-specific antibody was approximately 40 microg ml-1 of rabbit antiserum. Enzyme-linked immunobsorbant assay inhibition studies using sugars, sugar alcohols and L-lysine showed minimal cross-reactivity (approximately 4%) when compared with erythritol; only dithioerythritol showed a cross-reactivity of approximately 33%. D-Threitol and L-threitol (isomers of erythritol) had cross-reactivities of 15 and 11%, respectively. The inhibition studies confirmed the haptenic nature of erythritol and indicated that the erythritoyl group is a single epitope. The reaction scheme outlined here for the generation of erythritol epitopes appears to provide a basis for the immunogenicity of erythritol.
K Sreenath; P Prabhasankar; Y P Venkatesh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food additives and contaminants     Volume:  23     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-11     Completed Date:  2007-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  England    
Other Details:
Languages:  eng     Pagination:  861-9     Citation Subset:  IM    
Department of Biochemistry & Nutrition, Central Food Technological Research Institute (CFTRI), Mysore, Karnataka State, India.
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MeSH Terms
Antibody Specificity*
Enzyme-Linked Immunosorbent Assay / methods
Erythritol / immunology*
Food Additives*
Haptens / immunology
Immune Sera / immunology
Immunization / methods
Serum Albumin, Bovine / immunology
Reg. No./Substance:
0/Food Additives; 0/Haptens; 0/Immune Sera; 0/Serum Albumin, Bovine; 149-32-6/Erythritol
Erratum In:
Food Addit Contam. 2006 Oct;23(10):1053

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