Document Detail


Generation of anti-trenbolone monoclonal antibody and establishment of an indirect competitive enzyme-linked immunosorbent assay for detection of trenbolone in animal tissues, feed and urine.
MedLine Citation:
PMID:  21147313     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Trenbolone (TRE) is a steroid used by veterinarians on livestock to increase appetite and body weight. The use of TRE has been restricted because of its harmful side effect for consumers. To effectively control TRE residue in food and food product, a rapid and convenient immunoassay was developed by preparing an anti-TRE monoclonal antibody. The immunogen and coating antigen were prepared by coupling TRE hapten with carrier proteins via 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC) method. The optimized method gave an average IC(50) value of 0.323ngmL(-1) towards TRE and an average detection limit (LOD) of 0.06ngmL(-1), which is much lower than the maximum residue levels (2.0ngg(-1)) accepted by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). The specificity of the antibody was evaluated by measuring cross-reactivity of six structurally related compounds, including 19-nortestosterone (9.7%), testosterone (0.13%), methyltestosterone (<0.01%), methandrostenolone (<0.01%), (+)-dehydroisoandrosterone (<0.001%) and β-estradiol (<0.001%). The recovery rates of the test in detection of TRE-fortified animal tissue, urine and animal feed samples were in the range of 81.3-89.4%, while the intra- and inter-assay coefficients of variation were less than 12.0%.
Authors:
Yuanyang Zhang; Fangyang He; Yuping Wan; Meng Meng; Jing Xu; Jian Yi; Yabin Wang; Caiwei Feng; Shanliang Wang; Rimo Xi
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Publication Detail:
Type:  Journal Article     Date:  2010-10-23
Journal Detail:
Title:  Talanta     Volume:  83     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  732-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
The School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, PR China.
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