Document Detail


Generation of affinity for antithrombin III by supplemental sulfation of heparin species with low affinity for the protein.
MedLine Citation:
PMID:  2292586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin III (Mr 7,500-18,000; anti-clotting activity, 7 USP units/mg), having degrees of sulfate substitution at D-glucosamine and L-iduronic acid residues of GlcNS 0.786, GlcN6s 0.628, and IdoA2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in N,N-dimethylformamide at -10 degrees C for 1 h. Both chemical and NMR spectroscopic analyses revealed that sulfation proceeded exclusively at HO-6 in D-glucosamine and HO-2 in L-iduronic acid residues, according to the amount of the sulfating reagent used (GlcNS: 0.825, 0.830; GlcN6s: 0.872, 0.928; IdoA2s: 0.687, 0.749 mol, respectively). Affinity chromatography of the sulfated products on antithrombin III-Sepharose gel indicated that the polysaccharide acquired some affinity for the protein following the sulfation, as shown by the increase in the proportion of the high-affinity heparin fraction (%) from 1.1 to 6.7. Biological examination of these products indicated that sulfation at natural positions along with the polysaccharide chain resulted in significant increases in all the activities (blood anti-clotting, anti-Factor IIa, and anti-Factor Xa), and in the strength of intrinsic fluorescence of antithrombin III.
Authors:
A Ogamo; A Metori; H Uchiyama; K Nagasawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  108     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-04-08     Completed Date:  1991-04-08     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  588-92     Citation Subset:  IM    
Affiliation:
School of Pharmaceutical Sciences, Kitasato University, Tokyo.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anticoagulants / metabolism
Antithrombin III*
Chromatography, Affinity
Chromatography, Gel
Heparin / metabolism*
Sulfates / metabolism*
Swine
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Sulfates; 9000-94-6/Antithrombin III; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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