Document Detail


Generation of active pools of abscisic acid revealed by in vivo imaging of water-stressed Arabidopsis.
MedLine Citation:
PMID:  15618419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A noninvasive, cell-autonomous reporter system was developed to monitor the generation and distribution of physiologically active pools of abscisic acid (ABA). ABA response (abi1-1) and biosynthesis (aba2-1) mutants of Arabidopsis (Arabidopsis thaliana) were used to validate the system in the presence and absence of water stress. In the absence of water stress, low levels of ABA-dependent reporter activation were observed in the columella cells and quiescent center of the root as well as in the vascular tissues and stomata of cotyledons, suggesting a nonstress-related role for ABA in these cell types. Exposure of seedlings to exogenous ABA resulted in a uniform pattern of reporter expression. In marked contrast, reporter expression in response to drought stress was predominantly confined to the vasculature and stomata. Surprisingly, water stress applied to the root system resulted in the generation of ABA pools in the shoot but not in the root. The analysis of the response dynamics revealed a spread of physiologically active ABA from the vascular tissue into the areoles of the cotyledons. Later, ABA preferentially activated gene expression in guard cells. The primary sites of ABA action identified by in planta imaging corresponded to the sites of ABA biosynthesis, i.e. guard cells and cells associated with vascular veins. Hence, water stress recognized by the root system predominantly results in shoot-localized ABA action that culminates in a focused response in guard cells.
Authors:
Alexander Christmann; Thomas Hoffmann; Irina Teplova; Erwin Grill; Axel Müller
Related Documents :
2685109 - Immunohistochemical localization of vimentin in the gerbil inner ear.
11541079 - Geoperception in primary and lateral roots of phaseolus vulgaris (fabaceae). i. structu...
18979169 - Tissue-specific expression of the ethylene biosynthetic machinery regulates root growth...
12777689 - Molecular mechanisms that regulate auditory hair-cell differentiation in the mammalian ...
10066269 - Immunocytochemical and morphological evidence for intracellular self-repair as an impor...
2310549 - A cytoskeletal spring for the control of cell shape in outer hair cells isolated from t...
10720959 - Sialic acid and crystal binding.
14729579 - Heparin/heparan sulfate interacting protein plays a role in apoptosis induced by antica...
11003689 - Control of cell ph in the t84 colon cell line.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-23
Journal Detail:
Title:  Plant physiology     Volume:  137     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-12     Completed Date:  2005-05-24     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  209-19     Citation Subset:  IM    
Affiliation:
Lehrstuhl für Botanik, Technische Universität München, D-85354 Freising, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / metabolism,  physiology*
Arabidopsis / metabolism*
Gene Expression Regulation, Plant
Osmotic Pressure
Plant Roots / metabolism
Plant Shoots / metabolism
Water / metabolism*
Chemical
Reg. No./Substance:
21293-29-8/Abscisic Acid; 7732-18-5/Water
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Male germ line development in Arabidopsis. duo pollen mutants reveal gametophytic regulators of gene...
Next Document:  Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts.