Document Detail

Generation of active pools of abscisic acid revealed by in vivo imaging of water-stressed Arabidopsis.
MedLine Citation:
PMID:  15618419     Owner:  NLM     Status:  MEDLINE    
A noninvasive, cell-autonomous reporter system was developed to monitor the generation and distribution of physiologically active pools of abscisic acid (ABA). ABA response (abi1-1) and biosynthesis (aba2-1) mutants of Arabidopsis (Arabidopsis thaliana) were used to validate the system in the presence and absence of water stress. In the absence of water stress, low levels of ABA-dependent reporter activation were observed in the columella cells and quiescent center of the root as well as in the vascular tissues and stomata of cotyledons, suggesting a nonstress-related role for ABA in these cell types. Exposure of seedlings to exogenous ABA resulted in a uniform pattern of reporter expression. In marked contrast, reporter expression in response to drought stress was predominantly confined to the vasculature and stomata. Surprisingly, water stress applied to the root system resulted in the generation of ABA pools in the shoot but not in the root. The analysis of the response dynamics revealed a spread of physiologically active ABA from the vascular tissue into the areoles of the cotyledons. Later, ABA preferentially activated gene expression in guard cells. The primary sites of ABA action identified by in planta imaging corresponded to the sites of ABA biosynthesis, i.e. guard cells and cells associated with vascular veins. Hence, water stress recognized by the root system predominantly results in shoot-localized ABA action that culminates in a focused response in guard cells.
Alexander Christmann; Thomas Hoffmann; Irina Teplova; Erwin Grill; Axel Müller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-23
Journal Detail:
Title:  Plant physiology     Volume:  137     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-12     Completed Date:  2005-05-24     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  209-19     Citation Subset:  IM    
Lehrstuhl für Botanik, Technische Universität München, D-85354 Freising, Germany.
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MeSH Terms
Abscisic Acid / metabolism,  physiology*
Arabidopsis / metabolism*
Gene Expression Regulation, Plant
Osmotic Pressure
Plant Roots / metabolism
Plant Shoots / metabolism
Water / metabolism*
Reg. No./Substance:
21293-29-8/Abscisic Acid; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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