Document Detail

Generation of active oxygen species in blood platelets--spin trapping analysis.
MedLine Citation:
PMID:  1314759     Owner:  NLM     Status:  MEDLINE    
Generation of active oxygen species by bovine blood platelets was examined by the electron spin resonance (ESR) spin trapping technique with 5,5-dimethyl-1-pyroline-1-oxide (DMPO). The hydroxyl spin-trapped adduct 5,5-dimethyl-2-hydroxyl-1-pyrolidinyloxy (DMPO-OH) was formed in the presence of platelets, indicating the generation of hydroxyl radicals (.OH) by the platelets. Generation of .OH was observed even with platelets in the resting state, but was markedly enhanced when the platelets were activated with stimulants. Stronger stimulants such as the calcium ionophore ionomycin, induced greater radical generation than the weaker stimulant ADP. When the platelets were stimulated by thrombin, generation of .OH was greatest after 1.5 min, and depended on the dose of the stimulant. It was inhibited by inhibitors of platelet activation such as forskolin and phenolic antioxidants.
S Kitagawa; H Fujisawa; F Kametani; H Sakurai
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Free radical research communications     Volume:  15     ISSN:  8755-0199     ISO Abbreviation:  Free Radic. Res. Commun.     Publication Date:  1992  
Date Detail:
Created Date:  1992-05-27     Completed Date:  1992-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709453     Medline TA:  Free Radic Res Commun     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  319-24     Citation Subset:  IM    
Niigata College of Pharmacy, Japan.
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MeSH Terms
Adenosine Diphosphate / pharmacology
Blood Platelets / metabolism*
Cyclic N-Oxides
Electron Spin Resonance Spectroscopy
Free Radicals
Hydroxides / blood
Hydroxyl Radical
Oxygen / blood*
Platelet Activation / drug effects,  physiology
Spin Labels
Thrombin / pharmacology
Reg. No./Substance:
0/Cyclic N-Oxides; 0/Free Radicals; 0/Hydroxides; 0/Spin Labels; 3317-61-1/5,5-dimethyl-1-pyrroline-1-oxide; 3352-57-6/Hydroxyl Radical; 58-64-0/Adenosine Diphosphate; 7782-44-7/Oxygen; EC

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