Document Detail


Generation of a three-dimensional ultrastructural model of human respiratory cilia.
MedLine Citation:
PMID:  22936404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ultrastructures of cilia and flagella are highly similar and well conserved through evolution. Consequently, Chlamydomonas is commonly used as a model organism for the study of human respiratory cilia. Since detailed models of Chlamydomonas axonemes were generated using cryoelectron tomography, disparities among some of the ultrastructural features have become apparent when compared with human cilia. Extrapolating information on human disease from the Chlamydomonas model may lead to discrepancies in translational research. This study aimed to establish the first three-dimensional ultrastructural model of human cilia. Tomograms of transverse sections (n = 6) and longitudinal sections (n = 9) of human nasal respiratory cilia were generated from three healthy volunteers. Key features of the cilium were resolved using subatomic averaging, and were measured. For validation of the method, a model of the well characterized structure of Chlamydomonas reinhardtii was simultaneously generated. Data were combined to create a fully quantified three-dimensional reconstruction of human nasal respiratory cilia. We highlight key differences in the axonemal sheath, microtubular doublets, radial spokes, and dynein arms between the two structures. We show a decreased axial periodicity of the radial spokes, inner dynein arms, and central pair protrusions in the human model. We propose that this first human model will provide a basis for research into the function and structure of human respiratory cilia in health and in disease.
Authors:
Thomas Burgoyne; Mellisa Dixon; Pradeep Luther; Claire Hogg; Amelia Shoemark
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-30
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  47     ISSN:  1535-4989     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-03     Completed Date:  2013-01-25     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  800-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Axoneme / ultrastructure
Chlamydomonas reinhardtii / ultrastructure
Cilia / ultrastructure*
Dyneins / metabolism,  ultrastructure
Electron Microscope Tomography
Humans
Imaging, Three-Dimensional*
Microtubules / metabolism,  ultrastructure
Models, Biological
Nasal Cavity / ultrastructure*
Grant Support
ID/Acronym/Agency:
RG/11/21/29335//British Heart Foundation
Chemical
Reg. No./Substance:
EC 3.6.4.2/Dyneins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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