Document Detail


Generation of Nitric Oxide and Reactive Oxygen Species by Neutrophils and Monocytes from Septic Patients and Association with Outcomes.
MedLine Citation:
PMID:  22575991     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: In this study we evaluated the reactive oxygen species (ROS) and nitric oxide (NO) generation by monocytes and neutrophils from septic patients and to correlate their levels with clinical outcomes. METHODS: Forty-nine septic patients and 19 healthy volunteers were enrolled in the study. The ROS and NO production was quantified in monocytes and neutrophils in whole blood by flow cytometry, constitutively and after stimulation with Staphylococcus aureus and Pseudomonas aeruginosa. RESULTS: NO production by monocytes was higher in SP compared with healthy volunteers for all conditions and by neutrophils at baseline, and ROS generation in monocytes and neutrophils was higher in septic patients than in healthy volunteers for all conditions. NO production by monocytes and neutrophils was decreased at D7 compared with D0 in survivors at baseline and after stimulation with S. aureus. ROS production by the monocytes and neutrophils was decreased in survivors at D7 compared with D0 under all conditions, except by neutrophils at baseline. No difference was found in NO and ROS generation by monocytes and neutrophils between D7 and D0 in non-survivors. CONCLUSIONS: Generation of NO and ROS by neutrophils and monocytes are increased in septic patients and their persistence is associated with poor outcome.
Authors:
Sidnéia Sousa Santos; Milena Karina Colo Brunialti; Otelo Rigato; Flavia Ribeiro Machado; Eliezer Silva; Reinaldo Salomao
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-9
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  -     ISSN:  1540-0514     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Division of Infectious Diseases, Department of Medicine, Hospital Sao Paulo, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil 2Critical Care Unit, Hospital Sirio-Libanes, Sao Paulo, Brazil 3Division of Anesthesiology, Department of Surgery, Hospital Sao Paulo, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil 4Critical Care Unit, Hospital Albert Einstein, Sao Paulo, Brazil.
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