Document Detail


Generation of an inducible mouse ES cell lines deficient for Argonaute proteins.
MedLine Citation:
PMID:  21528461     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Argonautes (Agos) are core effectors of RNA silencing. In several nonmammalian organisms, multiple Agos are known to exhibit specialized functions for distinct RNA silencing pathway. Mammals have four closely related Agos. To examine the functions of mammalian Agos in the microRNA silencing pathway, we generated mouse embryonic stem (ES) cells that are nullizygous for all Agos. This chapter describes a variety of techniques including BAC recombineering, gene targeting, and inducible Cre-loxP recombination, used to generate inducible Ago knock-out ES cells. The Ago-deficient ES cells provide an important tool for the study of mammalian RNA silencing.
Authors:
Hong Su; Xiaozhong Wang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  725     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-04-29     Completed Date:  2011-08-11     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-313     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Northwestern University, Evanston, IL, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Chromosomes, Artificial, Bacterial / genetics,  metabolism
Embryonic Stem Cells / metabolism*
Estrogen Antagonists / pharmacology
Eukaryotic Initiation Factors / deficiency*,  genetics
Gene Order
Gene Targeting
Genetic Vectors / genetics,  metabolism
Integrases / metabolism
Mice
Molecular Biology / methods*
Promoter Regions, Genetic / drug effects*,  genetics
Recombination, Genetic / genetics
Research Design
Tamoxifen / analogs & derivatives,  pharmacology
Grant Support
ID/Acronym/Agency:
5R21GM079528/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Estrogen Antagonists; 0/Eukaryotic Initiation Factors; 10540-29-1/Tamoxifen; 17197F0KYM/afimoxifene; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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