Document Detail


Generation of Human Bronchial Epithelial Cell Lines Expressing Inactive Mutants of GALNT3.
MedLine Citation:
PMID:  22785053     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
As a tool to understand the role of mucins in the infection of respiratory viruses, we established cell lines stably expressing inactive mutants of UDP-GalNAc:polypeptide N-acetylgalactosaminlytransferase 3 (GALNT3), which initiates O-glycosylation of mucins. We introduced single-amino-acid mutations into the regions essential for the enzyme activity of GALNT3 using the expression plasmid of human GALNT3 and transfected the mutant constructs into the human bronchial epithelial cell line, BEAS-2B. We showed that although the mutants of GALNT3 exhibit an authentic localization at the Golgi apparatus, the glycosylation pattern of the expressing cell lines is appeared to be different from that of the cells expressing wild-type GALNT3. These results suggested that the established cell lines express inactive forms of GALNT3 and might be useful to investigate the significance of O-glycosylation of mucins in respiratory virus infections.
Authors:
Shoko Nakamura; Masayuki Horie; Kan Fujino; Yusuke Matsumoto; Tomoyuki Honda; Keizo Tomonaga
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-12
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  -     ISSN:  1347-7439     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Viral Oncology, Institute for Virus Research, Kyoto University.
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