Document Detail


Generation of homogeneous PDX1(+) pancreatic progenitors from human ES cell-derived endoderm cells.
MedLine Citation:
PMID:  19910415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One key step in producing insulin-secreting cells from human embryonic stem (hES) cells is the generation of pancreatic and duodenal homeobox gene 1 (PDX1)-expressing pancreatic progenitor cells. All-trans retinoic acid (RA) has important roles in pancreas development and is widely used to induce pancreatic differentiation of ES cells. When RA was added directly to the activin A-induced hES cells, <20% cells were positive for the pancreatic marker PDX1, whereas the other cells were mainly hepatic cells. We found that when the activin A-induced hES cells were replated and seeded at low cell densities, the addition of RA induced significant pancreatic differentiation and over 70% of cells in culture expressed PDX1. When the endodermal cells were isolated with the surface marker CXCR4 from the activin A-induced culture and further differentiated with RA, a homogeneous PDX1(+) cell population (over 95% pure) was generated. The PDX1(+) cells could further differentiate into cells that expressed pancreatic transcription factors and pancreatic endocrine or exocrine markers. We also found that RA inhibited the hepatic differentiation of endodermal cells that were seeded at low cell densities, and this inhibition may have been through the inhibition of Smad1/5/8 activity. Thus, we present a highly efficient and reproducible protocol for generating PDX1(+) pancreatic progenitor cells from hES cells.
Authors:
Jun Cai; Chen Yu; Yanxia Liu; Song Chen; Yuxuan Guo; Jun Yong; Wei Lu; Mingxiao Ding; Hongkui Deng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-12
Journal Detail:
Title:  Journal of molecular cell biology     Volume:  2     ISSN:  1759-4685     ISO Abbreviation:  J Mol Cell Biol     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-20     Completed Date:  2010-03-29     Revised Date:  2011-12-29    
Medline Journal Info:
Nlm Unique ID:  101503669     Medline TA:  J Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  50-60     Citation Subset:  IM    
Affiliation:
The MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques*
Cell Differentiation*
Cells, Cultured
Embryonic Stem Cells / cytology*,  metabolism
Endoderm / cytology*,  metabolism
Homeodomain Proteins / genetics,  metabolism*
Humans
Insulin-Secreting Cells / cytology,  metabolism
Pancreas / cytology,  metabolism*
Trans-Activators / genetics,  metabolism*
Tretinoin / metabolism
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein; 302-79-4/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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