Document Detail


Generation of histocompatible androgenetic embryonic stem cells using spermatogenic cells.
MedLine Citation:
PMID:  20020425     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Androgenetic embryonic stem (aES) cells, produced by pronuclear transplantation, offer an important autologous pluripotent stem cell source. However, the isolation of aES cells, particularly individual-specific aES cells, with the use of fertilized embryos has limited the practical applications of this technology in humans. In this study, we applied a new approach, essentially described as somatic cell nuclear transfer, and generated three aES cell line types with the use of spermatogenic cells including primary spermatocytes, round spermatids, and mature spermatozoa as donor cells, omitting the need to use fertilized embryos. Although abnormality of chimeras and absent germline competency indicated that all three types of aES cells exhibited limited pluripotency, the epigenetic status of the aES cell lines tended to resemble normal ES cells during long-term culture, and some parental-specific imprinted genes were expressed at levels comparable to those of normal ES cells. Furthermore, the histocompatibility of the aES cells was investigated by transplanting the differentiation progenies of the aES cells into major histocompatibility (MHC)-matched and -mismatched recipient mice. The results indicated that these aES cells were histocompatible with MHC-matched mice after transplantation. Our study provides evidence that MHC-competent autologous aES cells could be generated from different spermatogenic cells using nuclear transfer into oocytes, a process that could avoid the use of fertilized embryos.
Authors:
Qingguo Zhao; Jianle Wang; Yu Zhang; Zhaohui Kou; Sheng Liu; Shaorong Gao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  28     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  229-39     Citation Subset:  IM    
Affiliation:
National Institute of Biological Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Cell Differentiation / physiology
Cells, Cultured
DNA Methylation
Embryonic Stem Cells / cytology*,  metabolism*
Immunohistochemistry
Male
Mice
Nuclear Transfer Techniques
Reverse Transcriptase Polymerase Chain Reaction
Spermatids / cytology,  metabolism
Spermatocytes / cytology,  metabolism
Spermatogenesis / genetics,  physiology*
Spermatogonia / cytology,  metabolism

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